郑州大学学报（医学版）2026，Vol.61Issue(3)：29-33,5.DOI:10.13705/j.issn.1671-6825.2025.03.047
基于Tim-3/Gal-9信号通路探讨艾灸对类风湿关节炎大鼠的治疗作用
Therapeutic effects of moxibustion on rheumatoid arthritis rats based on Tim-3/Gal-9 signaling pathway
摘要
Abstract
Aim:To investigate the therapeutic effects of moxibustion on rheumatoid arthritis(RA)rats based on the T cell immunoglobulin and mucin domain-containing protein 3/galectin-9(Tim-3/Gal-9)signaling pathway.Methods:Twen-ty-four SD rats were randomly divided into control group,model group,moxibustion group,and sh-Tim-3+moxibustion group,with 6 rats in each group.RA was induced by subcutaneously injecting 100 μL of 0.5 mg/mL Freund's complete ad-juvant into the foot pads of both hind paws.Seven days later,rats in the moxibustion group received moxibustion at Shenshu(BL23)and Zusanli(ST36)acupoints 5 times daily.The sh-Tim-3+moxibustion group was injected with sh-Tim-3 lentivir-us expression vector into the bilateral hind foot pads,and moxibustion was performed 1 hour later.All treatments performed 6 days per week for 3 weeks.After the intervention,the foot pad thickness was measured.Serum levels of TNF-α,IL-6,IL-12,IL-13,and TGF-β were detected by ELISA.Histological changes of the ankle synovial tissue were observed by HE stai-ning,and pathological scoring was performed.Macrophage phenotypes in the synovial tissue were assessed by immunofluo-rescence.Tim-3 and Gal-9 mRNA expression in the spleen and synovial tissues were detected by real-time quantitative PCR.Tim-3 and Gal-9 protein expression in the synovial tissue were detected by Western blot.Results:Compared with the control group,the model group exhibited increased footpads thickness on days 7,14,21 and 28,elevated serum levels of TNF-α,IL-6,and IL-12,and a higher synovial iNOS+/CD206+ratio(P＜0.05).Conversely,serum levels of IL-13 and TGF-β,Tim-3 and Gal-9 mRNA expression in the spleen and synovial tissues,as well as Tim-3 and Gal-9 protein expression in the synovial tissue decreased(P＜0.05).Compared with the model group,the changes in the above indexes of the moxi-bustion group were opposite(P＜0.05).The lentivirus weakened the effects of moxibustion(P＜0.05).Conclusion:Moxi-bustion may regulate macrophage polarization through the Tim-3/Gal-9 signaling pathway,enhance the anti-inflammatory ca-pacity,and alleviate the clinical symptoms of RA rats.关键词
T细胞免疫球蛋白黏蛋白域蛋白3/半乳凝素-9/艾灸/类风湿关节炎/大鼠Key words
T cell immunoglobulin and mucin domain-containing protein 3/galectin-9/moxibustion/rheumatoid arthri-tis/rat分类
医药卫生引用本文复制引用
徐溢梅,段玉松,聂敬伟,黄俊,崔露,黄志灿,周海燕..基于Tim-3/Gal-9信号通路探讨艾灸对类风湿关节炎大鼠的治疗作用[J].郑州大学学报（医学版）,2026,61(3):29-33,5.基金项目
国家自然科学基金项目(82374587,81973959) （82374587,81973959）
四川省自然科学基金项目(2024NSFSC2113) （2024NSFSC2113）
