甘蔗糖业2026，Vol.55Issue(3)：83-90,8.DOI:10.3969/j.issn.1005-9695.2026.03.009
酶辅助-超声协同提取甘蔗多酚工艺条件优化
Optimization of Process Conditions for Enzyme-Assisted-Ultrasonic Synergistic Extraction of Sugarcane Polyphenols
摘要
Abstract
Using sugarcane rind as raw material,polyphenols were extracted by enzyme-assisted ultrasonic synergistic extraction(EASE).On the basis of single-factor experiments,the Box-Behnken response surface methodology was employed to optimize four key process parameters:cellulase dosage,ultrasonic power,extraction temperature,and ultrasonic time.Analysis of variance showed that the influence of the factors on polyphenol yield followed the order of ultrasonic power＞cellulase dosage＞extraction temperature＞ultrasonic time,and the established quadratic polynomial regression model exhibited a good fit(R2=0.9746).The optimal process conditions were cellulase dosage of 0.45％,ultrasonic power of 350 W,extraction temperature of 52 ℃,and ultrasonic time of 35 min.Under these conditions,the polyphenol yield reached 4.79 mg/g±0.05 mg/g.Compared with traditional heat reflux extraction and single ultrasound-assisted extraction,the synergistic extraction significantly shortened the extraction time,and increased the polyphenol yield by 28.5％and 12.3％,respectively;the DPPH radical scavenging activity was also improved.The complementary effects between enzymatic cell wall disruption and ultrasonic cavitation-enhanced mass transfer effectively promoted the release of sugarcane rind polyphenols.This study provides technical support for the high-value utilization of sugarcane processing by-products and the development of functional food ingredients.关键词
甘蔗多酚/酶辅助提取/超声波/Box-Behnken设计/响应面法/抗氧化活性Key words
Sugarcane polyphenols/Enzyme-assisted extraction/Ultrasound/Box-Behnken design/Response surface methodology/Antioxidant activity分类
轻工纺织引用本文复制引用
宁方尧,陈延,黄凯,温韬..酶辅助-超声协同提取甘蔗多酚工艺条件优化[J].甘蔗糖业,2026,55(3):83-90,8.基金项目
广西教育厅2025年职业教育教学改革项目(GXGZJG2025A010) （GXGZJG2025A010）
广西工业职业技术学院2024年度第一批校级科研项目(GYKY2024001A) （GYKY2024001A）
广西工业职业技术学院2025年度第一批校级教科研项目(GYJG2025008A) （GYJG2025008A）
