郑州大学学报（医学版）2026，Vol.61Issue(3)：169-172,4.DOI:10.13705/j.issn.1671-6825.2025.10.009
宏基因组二代测序对肺炎克雷伯杆菌碳青霉烯耐药性的诊断价值
Diagnostic value of metagenomic next-generation sequencing for carbap-enem resistance in klebsiella pneumoniae
王海旭 1赵颖颖 1胡旗旗 2曹萌萌 3杜雨新 4时学秀 1赵楠 1张雅文1
作者信息
- 1. 郑州大学第一附属医院综合 ICU 郑州 450052
- 2. 永煤集团总医院 河南 商丘 476600
- 3. 郑州大学第一附属医院重症医学规培基地 郑州 450052
- 4. 南阳市中心医院 河南 南阳 473000
- 折叠
摘要
Abstract
Aim:To explore the diagnostic value of metagenomic next-generation sequencing(mNGS)for carbapenem resistance in Klebsiella pneumoniae.Methods:A retrospective analysis was conducted on biological sample data from infec-ted patients who underwent both mNGS testing and traditional bacterial culture in the Intensive Care Unit of the First Affilia-ted Hospital of Zhengzhou University from May 2021 to March 2024.Using the antimicrobial susceptibility test results of bacterial culture as the gold standard,the detection results of both methods were compared,and an ROC curve was plotted to evaluate the diagnostic efficacy of mNGS.Results:The consistency of mNGS detection results for the drug-resistant gene blaKPC with the gold standard was good[Kappa(95%CI)=0.873(0.817-0.928)].The detection results of blaCTX-M and blaTEM genes showed moderate consistency with the gold standard[Kappa(95%CI)=0.414(0.311-0.518)and 0.419(0.316-0.521),respectively].blaKPC exhibited the best diagnostic performance,with an AUC(95%CI)of 0.94(0.91-0.97),a sensitivity of 92.8%,a specificity of 94.8%,and an accuracy of 93.7%.Conclusion:mNGS can rapidly identify resistance genes in carbapenem-resistant Klebsiella pneumoniae,enabling early detection of carbapenem resistance in Klebsiella pneumoniae.关键词
宏基因组二代测序/耐药基因/肺炎克雷伯菌/耐碳青霉烯Key words
metagenomic next-generation sequencing/drug resistance genes/Klebsiella pneumoniae/carbapenem resist-ance分类
医药卫生引用本文复制引用
王海旭,赵颖颖,胡旗旗,曹萌萌,杜雨新,时学秀,赵楠,张雅文..宏基因组二代测序对肺炎克雷伯杆菌碳青霉烯耐药性的诊断价值[J].郑州大学学报（医学版）,2026,61(3):169-172,4.
