湖南中医药大学学报2026，Vol.46Issue(5)：873-878,6.DOI:10.3969/j.issn.1674-070X.2026.05.001
通阳活血方对慢性心力衰竭大鼠心功能和血清炎症因子TNF-α、IL-6的影响
Effects of Tongyang Huoxue Formula on cardiac function and serum inflammatory factors TNF-α and IL-6 in rats with chronic heart failure
摘要
Abstract
Objective To observe the effects of Tongyang Huoxue Formula(TYHXF)on cardiac function and serum inflamm-atory factors TNF-α and IL-6 in rats with chronic heart failure(CHF),and to explore the possible mechanism of action of TYHXF in treating CHF.Methods Fifty-two SD rats were randomly divided into sham operation group(n=10),and operation group(n=42).The CHF model was established in the surgical group by abdominal aortic constriction.After successful modeling,rats were randomly assigned into model,TYHXF,and bisoprolol groups,with 14 rats in each group.The TYHXF group received TYHXF granule(21.25 g·kg-1·d-1)by gavage;the bisoprolol group received bisoprolol fumarate oral suspension(1 mg·kg-1·d-1)by gavage;and both the sham operation and model groups received an equal volume of distilled water by gavage.Gavage administration started one week after modeling,and continued once daily until eight consecutive weeks.At the end of the 16-week experimental period after modeling,echocardiography was performed,levels of serum N-terminal pro-B-type natriuretic peptide,TNF-α and IL-6,and cardiac mass index were measured.Histopathological changes in myocardial tissue were observed by HE and Masson staining.Results Compared with the sham operation group,rats in the model group exhibited increased left ventricular end-systolic diameter(LVESD)and left ventricular end-diastolic diameter(LVEDD)(P<0.05),decreased left ventricular ejection fraction(LVEF)and left ventricular fractional shortening(LVFS)(P<0.05),elevated levels of NT-proBNP,TNF-α and IL-6(P<0.05,P<0.01),and increased cardiac mass index(P<0.05).Compared with the model group,both the TYHXF and bisoprolol groups showed reduced LVESD and LVEDD(P<0.05),increased LVEF(P<0.05),decreased NT-proBNP levels(P<0.05,P<0.01),and decreased cardiac mass index(P<0.05).The TYHXF group showed reduced levels of TNF-α and IL-6(P<0.05,P<0.01),and the bisoprolol group showed reduced IL-6 levels(P<0.01).Conclusion TYHXF can alleviate myocardial hypertrophy,improve myocardial fibrosis,reduce serum NT-proBNP levels,improve cardiac function,and inhibit the overexpression of serum inflammatory factors TNF-α and IL-6 in rats with CHF.关键词
慢性心力衰竭/通阳活血方/氨基末端脑利钠肽前体/肿瘤坏死因子-α/白细胞介素-6Key words
chronic heart failure/Tongyang Huoxue Formula/N-terminal pro-B-type natriuretic peptide/tumor necrosis factor-α/interleukin-6分类
医药卫生引用本文复制引用
汪艳丽,任甜甜,李翠芝,刘金凤,关宣可,吴巧敏,常兴,刘如秀..通阳活血方对慢性心力衰竭大鼠心功能和血清炎症因子TNF-α、IL-6的影响[J].湖南中医药大学学报,2026,46(5):873-878,6.基金项目
中央高水平中医医院临床研究和成果转化能力提升项目(HLCMHPP20230118) （HLCMHPP20230118）
国家自然科学基金青年基金项目(81904045) （81904045）
院级优秀青年科技人才专项(ZZ14-YQ-014) （ZZ14-YQ-014）
中国中医科学院学部委员学术传承与传播专项项目(CI2022E012XB) （CI2022E012XB）
中央高水平中医医院临床研究和成果转化能力提升项目(HLCMHPP2023053) （HLCMHPP2023053）
北京中医药薪火传承"新 3+3"工程刘志明门人(刘如秀)传承工作站(2023-SZ-G-02) （刘如秀）
北京中医药薪火传承"新 3+3"工程刘志明"三名"传承工作室(2023-SZ-F-06). （2023-SZ-F-06）
