核农学报2026，Vol.40Issue(7)：1321-1330,10.DOI:10.11869/j.issn.1000-8551.2026.07.1321
小麦GPX基因鉴定、表达与自然等位变异分析
Identification,Expression and Natural Allelic Variation Analysis of Wheat GPX Genes
摘要
Abstract
Enhancing glutathione peroxidase(GPX)activity is a promising strategy for developing selenium-enriched wheat(Triticum aestivum L.).To identify members of the wheat GPX gene family and characterize their expression patterns across tissues,developmental stages,and selenium(Se)treatments,this study integrated bioinformatics and molecular biology approaches to systematically characterize e the structure,physicochemical properties,and functional expression of TaGPX genes.Fourteen GPX genes were detected in common wheat,unevenly distributed across chromosomes 2,4,6,and 7 of the A,B,and D homologous groups.Comparative analysis showed that TaGPX genes located on homologous chromosomes were predominantly localized to chloroplasts and chromosomes,with 5 to 6 exons and similar motif numbers.Promoter cis-acting element prediction indicated that most aGPX genes harbor abundant regulatory elements responsive to abiotic stress.By constructing a GPX evolutionary tree,it was found that wheat GPX genes are most closely related to those of Aegilops tauschii,with high similarity in gene differentiation.Transcriptome data showed that most TaGPX genes are highly expressed in leaves,with the highest expression levels at the flag leaf and wax ripening stage,suggesting the involvement of this gene family in scavenging reactive oxygen species(ROS)clearance within chloroplasts.The expression level of TaGPX genes in leaves was significantly higher than in seeds.Integrative analysis of genomic variation and transcriptome profiles implied that the SNP variations in TaGPX genes may contribute to improved ROS detoxification capacity in wheat system itself.Quantitative expression analysis across tissues and selenium salt treatment showed that TaGPX3.2A had the highest relative expression level.It was speculated that sulfur would compete with selenium for absorption sites under selenium supplementation,thereby limiting selenium absorption.De-sulfurization combined with selenium application could increase the expression level of TaGPX genes.This study laid a foundation for leveraging TaGPX genes to improve selenium biofortification in wheat.关键词
普通小麦(Triticum aestivum L.)/谷胱甘肽过氧化物酶/生物信息分析/非生物胁迫Key words
common wheat(Triticum aestivum L.)/glutathione peroxidase/bioinformatics analysis/abiotic stress引用本文复制引用
王新宇,王伟,郭子浩,史小霞,郝昱宁,韦小龙,吕晋慧,张春来..小麦GPX基因鉴定、表达与自然等位变异分析[J].核农学报,2026,40(7):1321-1330,10.基金项目
国家自然科学基金项目(31971944),山西省教育厅研究生教育创新计划实践创新项目(2023SJ109),山西省教育厅专业建设项目(J20220211),山西农谷建设科研专项(SXNGJSKYZX201702) （31971944）
