渔业科学进展2026，Vol.47Issue(3)：116-125,10.DOI:10.3969/j.issn.2095-9869.20250521001
17α-甲基睾酮对钝吻黄盖鲽生长和性腺发育的影响
Effects of 17α-Methyltestosterone on Growth and Gonadal Development of Pseudopleuronectes yokohamae
摘要
Abstract
The marbled flounder(Pseudopleuronectes yokohamae),characterized by significant female growth superiority,presents a compelling case for all-female aquaculture to address industry constraints in growth performance.Capitalizing on the well-documented XX/XY sex determination system,this study focused on developing an optimized pseudo-male(XX♂)induction protocol using 17α-methyltestosterone(MT)to establish monosex female populations for commercial-scale cultivation.Through a 120-day feeding trial with 60-day post hatching diploid juveniles(initial biometrics:1.48±0.27 cm body length,0.07±0.04 g body weight),we systematically evaluated five MT dosage regimens(0-4 mg/kg diet)for their sexual manipulation efficacy and associated biological impacts.Notably,all MT-treated groups achieved complete masculinization(100％),contrasting with the natural 50.0％±6.7％male ratio in the controls(MT0:7.61±0.72 cm,14.22±4.71 g).Growth metrics at 240 days revealed dose-dependent responses:Low-dose groups(MT0.5,and MT1)maintained growth and survival rates(64.0％±2.0％)comparable to those of the controls(P＞0.05),whereas high-dose treatments(MT2/MT4)significantly suppressed growth(P＜0.05).Histological analyses demonstrated preserved testicular microstructure in the MT0.5/MT1 groups,featuring distinct spermatogenic lobules and diverse spermatocyte stages,whereas high-dose groups exhibited structural disorganization with diminished spermatocyte populations.Molecular profiling of the meiosis marker sycp3 validated this dichotomy,showing peak expression in the MT1 group and significant downregulation in the MT2/MT4 groups relative to the controls(P＜0.05),confirming dose-mediated meiotic inhibition.These integrated findings establish 0.5 mg/kg MT as the optimal dosage,successfully reconciling complete sex reversal(100％masculinization)with uncompromised growth performance.This protocol provides a scientifically validated framework for industrial-scale all-female seedling production,effectively addressing zootechnical and reproductive management challenges in marbled flounder aquaculture.关键词
17α-甲基睾酮/钝吻黄盖鲽/伪雄鱼/性腺发育Key words
17α-Methyltestosterone/Pseudopleuronectes yokohamae/Pseudo-male fish/Gonadal development分类
农业科技引用本文复制引用
成路遥,范鑫,孟振,徐文腾,徐永江,张建设,张宁,徐荣静..17α-甲基睾酮对钝吻黄盖鲽生长和性腺发育的影响[J].渔业科学进展,2026,47(3):116-125,10.基金项目
国家重点研发计划(2022YFD2400402)、国家科技创新2030(2023ZD0405505)、国家海水鱼产业技术体系(CARS-047-G31)和中国水产科学研究院中央级公益性科研院所基本科研业务费专项资金(2025XT0502)共同资助. （2022YFD2400402）
