渔业科学进展2026，Vol.47Issue(3)：174-185,12.DOI:10.3969/j.issn.2095-9869.20250418003
福建养殖刺参腐皮综合征潜在致病菌的分离鉴定及耐药风险评估
Isolation,Identification and Drug Resistance Risk Assessment of Potential Pathogens Causing Skin Rot Syndrome in Apostichopus japonicus in Fujian Province
摘要
Abstract
In order to analyze the pathogenicity and drug resistance risk assessment of Apostichopus japonicus in Fujian province,the pathogen isolation,identification and pathogenicity analysis were carried out in 4 cases of A.japonicus in the Xiapu sea area in 2023.The antibiotic sensitivity and drug resistance risk assessment of corresponding bacterial pathogens were further carried out.In this study,a total of 4 dominant strains of 2335FTBA,231124FTBA,231210FTBA and 231214FTBA were isolated from the skin tissues of 4 outbreaks of the syndrome in the waters of Makang,Leijiang and Tanyu in Xiapu.Four strains of bacteria were identified as Vibrio atlanticus,Vibrio neocaledonicus and Vibrio atypicus by morphological analysis,16S rDNA sequencing,physiological and biochemical characteristics analysis and phylogenetic tree construction.Artificial infection showed that these four dominant strains were pathogenic to skin ulcer syndrome of A.japonicus.Their half-lethal concentrations(LC50)were 6.19×105,6.19×104,9.21×104 and 1.09×105 CFU/mL,respectively,showing strong pathogenicity.The results of drug sensitivity test showed that the multiple antibiotic resistance index(MARI)of the four strains were 0.35,0.76,0.62 and 0.59,respectively,indicating a high risk of antibiotic exposure.Combined with the relevant national drug management regulations and drug sensitivity test results,antibiotics containing doxycycline and flufenicol can be used for the treatment of corresponding diseases.The results of drug resistance gene detection of the 4 dominant strains showed that all the 4 dominant strains carried qnrA,qnrS and cmle3 genes,but did not carry floR genes.Except chloramphenicol resistance genes cmle3 and floR genes,other resistance genes carried the same drug resistance phenotype,indicating that the resistance genes had a strong correlation with the resistance phenotype.The relevant results can provide scientific basis for the analysis of pathogen diversity and disease prevention and control of Apostichopus japonicus.关键词
刺参/潜在致病菌/半致死浓度/抗生素耐药性/多重抗生素耐药性指数Key words
Apostichopus japonicus/Potential pathogenic bacteria/Semi-lethal concentration/Antibiotic resistance/Multiple antibiotic resistance index分类
农业科技引用本文复制引用
江敏棋,宋志伟,王锦锦,廖梅杰,荣小军,葛建龙,臧佳欣,于瀚哲,李彬,王印庚..福建养殖刺参腐皮综合征潜在致病菌的分离鉴定及耐药风险评估[J].渔业科学进展,2026,47(3):174-185,12.基金项目
宁德市"揭榜挂帅"关键核心技术攻关项目(ND2024J010)、中国水产科学研究院中央级公益性科研院所基本科研业务费专项资金(2023TD29)和农业农村部东海海水健康养殖重点实验室开放课题(2022ESHML03)共同资助. （ND2024J010）
