军事医学2026，Vol.50Issue(5)：321-333,13.DOI:10.7644/j.issn.1674-9960.2025-00270
短期3.5 GHz微波辐射致识别记忆障碍大鼠前额叶皮质转录组学研究
Transcriptomics study of the prefrontal cortex in rats with recognition memory impairment induced by short-term 3.5 GHz microwave radiation
田含科 1岳含霖 2南秀锋 1师博洋 1董霁 1赵黎 1王惠 1王浩宇 1彭瑞云1
作者信息
- 1. 军事科学院军事医学研究院,北京 100850
- 2. 中国科学院生物物理研究所,北京 100101
- 折叠
摘要
Abstract
Objective To investigate the effects of short-term 3.5 GHz microwave radiation on recognition memory and the gene expression profile of the prefrontal cortex of rats.Methods The behavioral performance of rats was evaluated via the novel object recognition(NOR)test,Morris water maze(MWM),and open field test(OFT)following short-term exposure to 3.5 GHz microwave radiation.Transcriptomic analysis was conducted to examine the alterations in gene expression profiles in the prefrontal cortex of rats after 6 days of 3.5 GHz microwave radiation.Key genes associated with microwave radiation induced recognition memory deficits were screened via gene interaction network analysis.The expression levels of the selected key genes were detected by real-time quantitative PCR(qPCR).Results After 6 days of 3.5 GHz microwave radiation,the recognition memory of rats significantly declined(P<0.05)while their spatial learning and memory remained unchanged,and no significant anxiety-like behaviors were observed.Compared with the Sham radiation group(Sham),1188 upregulated genes and 732 downregulated genes were detected in the prefrontal cortex of rats in the microwave radiation group(MW).Five key genes associated with microwave radiation-induced recognition memory impairment were identified by gene interaction network analysis.qPCR validation confirmed that in the prefrontal cortex,the expression levels of adrenoceptor beta 1(Adrb1),glutamate ionotropic receptor NMDA type subunit 2b(Grin2b),Kalirin Rho guanine nucleotide exchange factor kinase(Kalrn),and myocyte enhancer factor 2c(Mef2c)were significantly upregulated(P<0.05)while that of corticotropin releasing hormone receptor 1(Crhr1)was significantly downregulated(P<0.05).Conclusion Six days of exposure to 3.5 GHz microwave radiation can induce recognition memory impairment in rats,which may be attributed to the dysregulated gene expressions related to receptors,ion channels,and synaptic structure and function within the prefrontal cortex.关键词
微波辐射/识别记忆/转录组学/前额叶皮质/大鼠Key words
microwave radiation/recognition memory/transcriptomics/prefrontal cortex/rat分类
医药卫生引用本文复制引用
田含科,岳含霖,南秀锋,师博洋,董霁,赵黎,王惠,王浩宇,彭瑞云..短期3.5 GHz微波辐射致识别记忆障碍大鼠前额叶皮质转录组学研究[J].军事医学,2026,50(5):321-333,13.
