军事医学2026，Vol.50Issue(5)：342-350,9.DOI:10.7644/j.issn.1674-9960.2026-00034
基于RNA-seq技术探索低氧复合炎症对小胶质细胞代谢-免疫调控网络的协同作用机制研究
Microglial metabolic-immune crosstalk regulated by hypoxia combined with inflammation based on RNA-seq
摘要
Abstract
Objective To investigate the regulatory effects of hypoxia combined with inflammation on microglial energy metabolism and explore the underlying synergistic mechanisms in order to provide novel targets for the prevention and treatment of high-altitude cerebral edema.Methods BV2 microglial cells were categorized into four experimental groups:control(Con),hypoxia(Hy,1%O2),lipopolysaccharide(LPS,0.1 μg/mL),and hypoxia combined with LPS(Hy-LPS).Cell viability was evaluated using the CCK-8 assay.The levels of nitric oxide(NO)and pro-inflammatory cytokines(IL-1β,IL-6,TNF-α)in the supernatant were determined via the Griess method and ELISA,respectively.Transcriptomic profiling was conducted via RNA sequencing(RNA-seq),followed by bioinformatic analyses including identification of differentially expressed genes(DEGs),Gene Ontology(GO),Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment,and Gene Set Enrichment Analysis(GSEA).Results Hy-LPS co-treatment significantly suppressed cell viability and synergistically promoted the release of NO and inflammatory cytokines compared to individual stimuli.Transcriptomic analysis revealed 2060 differentially expressed genes(DEGs)in the Hy-LPS group,including 912 up-regulated and 1148 down-regulated genes.These DEGs were prominently enriched in pathways associated with hypoxia response,inflammatory immunity,cell cycle regulation,and metabolic reprogramming,indicating a robust synergistic effect between hypoxia and inflammation at the transcriptional level.Conclusion This study sheds light on the molecular network through which hypoxia complicated with inflammation synergistically regulates microglial energy metabolism and inflammatory responses across multiple pathways,thereby providing potential targets for mechanistic research and the prevention of high-altitude cerebral edema.关键词
低氧/神经炎症/高原脑水肿/小胶质细胞/转录组学/差异表达基因/代谢重编程Key words
hypoxia/neuroinflammation/high altitude cerebral edema/microglia/transcriptomics/differentially expressed genes/metabolic reprogramming分类
医药卫生引用本文复制引用
包素雅,张明喆,刘文惠,郭馨蔚,邢微微,高波,张安..基于RNA-seq技术探索低氧复合炎症对小胶质细胞代谢-免疫调控网络的协同作用机制研究[J].军事医学,2026,50(5):342-350,9.基金项目
重庆市科技局基金项目(CSTB2024TIAD-GPX0030) （CSTB2024TIAD-GPX0030）
