军事医学2026，Vol.50Issue(5)：358-366,9.DOI:10.7644/j.issn.1674-9960.2026-00039
柴陷花参汤化学成分及其解热镇痛活性部位的研究
Chemical components and antipyretic and analgesic active fractions of Chaixian Huashen decoction
摘要
Abstract
Objective To identify the chemical constituents of different fractions of the water extract of Chaixian Huashen decoction(CXHSD)and to evaluate the antipyretic and analgesic activities of each fraction in order to improve the extraction process.Methods The water extract of CXHSD was obtained via water reflux extraction(CXHSD-W).An alcohol-water extract was obtained via 70%ethanol reflux extraction once,followed by water reflux extraction twice(CXHSD-EW).The water extract was separated using macroporous adsorption resin into four elution fractions:water(CXHSD-00),30%ethanol(CXHSD-30),60%ethanol(CXHSD-60),and 95%ethanol(CXHSD-95).The constituents of each fraction were identified using UPLC-Q-TOF-MS technology against a self-built compound library.The contents of total flavonoids,total saponins,and total alkaloids in the water extract,the four elution fractions,and the alcohol-water extract were determined by ultraviolet spectrophotometry.The antipyretic and analgesic activities of each fraction were evaluated using a lipopolysaccharide(LPS)-induced fever model and an acetic acid-induced writhing pain model in mice.Results A total of 114 compounds were identified from the four fractions of the water extract.The main active compo-nents,including flavonoids,saponins,and alkaloids,were primarily distributed in CXHSD-30 and CXHSD-60.The results of ultraviolet spectrophotometric determination for total flavonoids,total saponins,and total alkaloids were consistent with the above findings.Animal experiments showed that CXHSD-60 significantly inhibited LPS-induced fever(P<0.001).The inhibition rate of CXHSD-30 was the highest(76.68%,P<0.001)against acetic acid-induced pain.CXHSD-EWcontained higher levels of total flavonoids and total saponins than CXHSD-W while possessing superior antipyretic and analgesic activities compared to CXHSD-W.Conclusion The addition of 70%ethanol extraction in CXHSD preparation increases the extraction yield of active substances and enhances its antipyretic and analgesic activities.The optimized extraction process for this formula is determined to be ethanol extraction once,followed by water extraction twice.关键词
柴陷花参汤/总黄酮/总皂苷/总生物碱/解热/镇痛Key words
Chaixian Huashen decoction/total flavonoids/total saponins/total alkaloids/antipyretic/analgesic分类
医药卫生引用本文复制引用
易毓晴,李长伟,李硕,张杨,杨郁,徐锐,麻浩,刘峰,肖智勇,张有志..柴陷花参汤化学成分及其解热镇痛活性部位的研究[J].军事医学,2026,50(5):358-366,9.基金项目
国家中医药管理局高水平中医药重点学科建设项目(zyyzdxk-2023311) （zyyzdxk-2023311）
