聊城大学学报（自然科学版）2026，Vol.39Issue(4)：603-613,11.DOI:10.19728/j.issn1672-6634.2025120017
咖啡酸苯乙酯抑制肺炎克雷伯菌增殖及生物膜形成的分子机制研究
Study on the molecular mechanism of caffeic acid phenethyl ester inhibiting the proliferation and biofilm formation of Klebsiella pneumoniae
摘要
Abstract
This study investigated the antibacterial activity of caffeic acid phenethyl ester(CAPE)against Klebsiella pneumoniae and explored its molecular mechanisms in inhibiting biofilm formation.The effects of CAPE on bacterial growth were evaluated by determining the minimum inhibitory concentration(MIC),minimum bactericidal concentration(MBC),and growth curves.Crystal violet staining,adhesion assays,and confocal laser scanning microscopy were used to assess the effects of CAPE on biofilm formation and structure.In addition,leakage of extracellular DNA and proteins,as well as changes in exopolysaccharides content,were quantitatively analyzed.RT-qPCR was performed to determine the expression levels of genes associated with biofilm formation,adhesion,and virulence.The results showed that the MIC and MBC of CAPE against K.pneumoniae were 0.312 5 mmol/L and 0.625 0 mmol/L,respectively,and that CAPE significantly inhibited bacterial growth.At 2 MIC,CAPE effectively removed mature biofilms,a-chieving a removal rate of 50.18%.Meanwhile,bacterial adhesion and exopolysaccharides content were reduced by 73.3%and 46.2%,respectively,and CAPE induced substantial leakage of extracellular DNA and proteins.RT-qPCR analysis further revealed significant downregulation of multiple genes related to biofilm formation.In conclusion,CAPE exhibits potent antibacterial activity against K.pneumoniae by impairing membrane integrity,inhibiting biofilm formation,and modulating the expression of virulence-related genes,supporting its potential as a natural antimicrobial agent.关键词
咖啡酸苯乙酯/肺炎克雷伯菌/抑菌活性/生物膜形成/分子机制Key words
caffeic acid phenethyl ester/Klebsiella pneumoniae/antibacterial activity/biofilm formation/molecular mechanisms分类
生物科学引用本文复制引用
谢瑛琪,高双双,李婷,李宗泽,玄红专..咖啡酸苯乙酯抑制肺炎克雷伯菌增殖及生物膜形成的分子机制研究[J].聊城大学学报（自然科学版）,2026,39(4):603-613,11.基金项目
泰山学者青年人工计划项目(tsqn202211172) （tsqn202211172）
山东省蜂产业技术体系产品加工与质量控制岗位(SDAIT-24-05)资助 （SDAIT-24-05）
