吉首大学学报（自然科学版）2026，Vol.47Issue(3)：50-59,10.DOI:10.13438/j.cnki.jdzk.2026.03.008
尿路感染病原体的四重荧光定量PCR检测技术
Quadruple Fluorescence PCR Detection Technology for Urinary Tract Infection Pathogens
摘要
Abstract
To rapidly identify and diagnose mixed infections of common pathogens in the urogenital tract,including Mycoplasma hominis,Ureaplasma urealyticum,Mycoplasma genitalium,and Chlamydia tra-chomatis,a quadruple fluorescence quantitative PCR technique was employed.Specific primers and fluo-rescence probes were designed based on the conserved gene regions of the four pathogens,and the reac-tion system was systematically optimized(primers concentration,probes concentration,and annealing temperature).Performance verification was also conducted.The results showed that the quadruple fluo-rescence quantitative PCR detection technique exhibited excellent specificity and high repeatability,with a detection sensitivity of up to 1000 copies/mL.Compared with conventional PCR technology,the four-step fluorescence quantitative PCR technique significantly improved the diagnostic efficiency and accuracy of mixed infections,and was suitable for rapid differential diagnosis of clinical mixed infections.关键词
人型支原体/解脲脲原体/生殖支原体/沙眼衣原体/四重荧光定量PCR检测技术Key words
Mycoplasma hominis/Ureaplasma urealyticum/Mycoplasma genitalium/Chlamydia tracho-matis/quadruple fluorescence PCR detection technology分类
医药卫生引用本文复制引用
邓欢,罗磊,刘佩珊,查满荣,邓中平,任小梅,吴康..尿路感染病原体的四重荧光定量PCR检测技术[J].吉首大学学报（自然科学版）,2026,47(3):50-59,10.基金项目
湖南省自然科学基金企业联合基金资助项目(2024JJ9149) （2024JJ9149）
