吉首大学学报（自然科学版）2026，Vol.47Issue(3)：60-66,7.DOI:10.13438/j.cnki.jdzk.2026.03.009
断血流的总黄酮提取工艺优化及质量评价
Optimization of Extraction Technology and Quality Evaluation of Total Flavonoids from Clinopodium polycephalum
摘要
Abstract
An orthogonal experiment was conducted to optimize the ethanol reflux extraction process of total flavonoids from Clinopodium polycephalum,and a stable method for determining the total fla-vonoid content was established.Based on this,quality evaluations were carried out on 6 batches of Cli-nopodium polycephalum medicinal materials from different sources.The results showed that the optimal extraction process was 70%ethanol volume fraction in 3 hours and 2 extractions;the RSD of precision tests,stability tests,repeatability tests,and sample recovery tests of the nitrous acid-sodium nitrate-sodi-um hydroxide colorimetric method were all less than 2%,indicating the accuracy and reliability of the method;the total flavonoid content of the 6 batches of Clinopodium polycephalum medicinal materials ranged from 0.067%to 0.154%,and the batch-to-batch coefficient of variation was as high as 32.72%.Even for medicinal material samples from the same origin,the total flavonoid content still showed signifi-cant differences,suggesting that the quality uniformity of Clinopodium polycephalum medicinal materi-als currently circulating in the online market is poor.关键词
断血流/总黄酮/乙醇回流提取/正交试验/质量评价/紫外可见分光光度法Key words
Clinopodium polycephalum/total flavonoids/ethanol reflux extraction/orthogonal test/quali-ty evaluation/ultraviolet-visible spectrophotometry分类
医药卫生引用本文复制引用
钟正午,刘楠,何佳敏,张婷..断血流的总黄酮提取工艺优化及质量评价[J].吉首大学学报（自然科学版）,2026,47(3):60-66,7.基金项目
湖南省教育厅优秀青年项目(23B0519) （23B0519）
