吉首大学学报（自然科学版）2026，Vol.47Issue(3)：67-72,6.DOI:10.13438/j.cnki.jdzk.2026.03.010
基于液-液色谱法分离黄花蒿凝血活性黄酮化合物
Flavonoid Compounds Separation with Coagulation Activity from Artemisia annua L.by Liquid-Liquid Chromatography
李淑惠 1罗江 1张晓蓉1
作者信息
- 1. 吉首大学药学院,湖南 吉首 416000
- 折叠
摘要
Abstract
A liquid-liquid chromatography method based on a spiral separation tube was developed for the separa-tion and purification of anthelmintic flavonoids from Artemisia annua L..To achieve the best separation effect,the mobile phase system and elution flow rate were systematically optimized,and HPLC analysis was used to fur-ther verify the separation effect.The results showed that the optimal mobile phase for the separated flavonoids from pH7,pH6,pH5,and pH4 samples was petroleum ether,petroleum ether-ethyl acetate(V(petroleum ether)∶V(ethyl acetate)=3∶1),petroleum ether-ethyl acetate(V(petroleum ether)∶V(ethyl acetate)=3∶1),and petroleum ether-ethyl acetate(V(petroleum ether)∶V(ethyl acetate)=1∶8),respectively;the optimal flow rate was 3 mL/min;after liquid-liquid chromatography separation of the flavonoids from pH7,pH6,and pH5 samples,a single component could be achieved;after separation of the flavonoids from pH4 sample,there were 4 components in the elution flow.It is suggested that the liquid-liquid chromatography based on the spiral separation tube can effectively separate the anthelmintic flavonoids from Artemisia annua L..关键词
黄花蒿/黄酮化合物/液-液色谱法Key words
Artemisia annua L./flavonoid compounds/liquid-liquid chromatography分类
医药卫生引用本文复制引用
李淑惠,罗江,张晓蓉..基于液-液色谱法分离黄花蒿凝血活性黄酮化合物[J].吉首大学学报（自然科学版）,2026,47(3):67-72,6.
