辽宁中医杂志2026，Vol.53Issue(6)：161-164,后插6,5.DOI:10.13192/j.issn.1000-1719.2026.06.039
柴胡皂苷d通过调控lncMEG3抗肝纤维化的机制研究
Mechanism Study of Saikosaponin-d Regulating lncMEG3 in Inhibiting Liver Fibrosis
摘要
Abstract
Objective To observe the effect of saikosaponin-d(SSd)on the expression of long noncoding RNA MEG3(ln-cRNA MEG3)in animal and cell models of liver fibrosis,and to explore the effect and mechanism of SSd on liver fibrosis.Meth-ods The expression of lncRNA MEG3 in liver tissue of rats with liver fibrosis induced by carbon tetrachloride(CCl4)was measured using qPCR.A lentiviral vector for interfering with lncMEG3 was constructed.The liver tissue was collected,and the pathological changes of liver fibrosis were examined using Masson and Sirius red staining.Adenovirus-mediated overexpression of lncMEG3 was transfected into TGF-β-induced LX-2 cells,and the effects of lncMEG3 overexpression on the expression levels of fibrotic phenotype α-smooth muscle actin(α-SMA)and collagen-related protein Collagen I were assessed via Western blot and qPCR.qPCR was used to detect the expression changes of lncMEG3 in primary hepatic stellate cells(HSCs)induced by CCl4 and LX-2 cells induced by transforming growth factor-β(TGF-β)treated with SSd.Results Compared with that in the control group,the expression of lncMEG3 was significantly down-regulated in the CCl4 induced liver fibrosis rat model,the extracted pri-mary hepatic stellate cells(HSCs)and TGF induced LX-2 cells.However,the treatment with SSd resulted in partial recovery of lncMEG3 expression.Compared with that in the control group,overexpression of lncMEG3 resulted in the down-regulation of the expressions of Collagen I and α-SMA protein as well as the mRNA expression levels of Collagen Ⅰ and α-SMA.SSd treatment significantly alleviated CCl4 induced liver fibrosis.After inhibiting lncMEG3 with a lentivirus that interferes with lncMEG3,the an-ti fibrotic effect of SSd was weakened.Conclusion SSd can inhibit collagen synthesis and anti liver fibrosis,and its mechanism may be related to the up-regulation of lncRNA MEG3 expression.关键词
lncRNA MEG3/胶原合成/肝纤维化/柴胡皂苷dKey words
lncRNA MEG3/collagen synthesis/liver fibrosis/saikosaponin-d分类
医药卫生引用本文复制引用
林柳兵,秦嫣,刘晓琳,张克慧,李勇..柴胡皂苷d通过调控lncMEG3抗肝纤维化的机制研究[J].辽宁中医杂志,2026,53(6):161-164,后插6,5.基金项目
国家自然科学基金青年项目(82304932) （82304932）
上海市中医药传承创新发展三年行动计划:海派中医非药物疗法推广体系建设项目[ZY(2025-2027)-3-2-1] （2025-2027）
