畜牧与饲料科学2026，Vol.47Issue(2)：107-115,9.DOI:10.12160/j.issn.1672-5190.2026.02.014
额敏县放牧羊寄生蜱体内沙门菌的分离鉴定、耐药性与毒力分析
Isolation,Identification,Drug Resistance and Virulence Analysis of Salmonella from Ticks Parasitizing Grazing Sheep in Emin County
摘要
Abstract
[Objective]To investigate the drug resistance and pathogenicity of Salmonella from ticks in grazing sheep in Emin County of Xinjiang Uygur Autonomous Region.[Methods]Ten live ticks were collected from the body surface of grazing sheep in Emin County.Morphological methods were used to identify the tick species.Bacteriological culture was employed to isolate the pathogenic bacteria in ticks.Morphological identification and molecular biology methods were applied to identify the isolated strains.The Kirby-Bauer disk diffusion method was used to perform drug susceptibility testing on the isolated strains.PCR amplification was employed to detect the carriage of drug resistance genes and virulence genes.[Results]Through morphological observation of ticks,10 ticks were all female Hyalomma asiaticum.Three strains of Salmonella were isolated from 10 ticks based on morphological observation,biochemical tests and molecular biological identification,and were designated as isolate 1,isolate 2 and isolate 3,respectively,with an isolation rate of 30.0％(3/10).The results of drug susceptibility testing showed that isolate 1 was resistant to streptomycin and meropenem,isolate 2 was resistant to meropenem,ertapenem and trimethoprim-sulfamethoxazole,and isolate 3 was resistant to amikacin and meropenem.All three isolates carried qnrS,qnrA,sul1,sul2 and aadA1 resistance genes.A total of 10 virulence genes were detected,and all isolates carried rck,spvB,sopE virulence genes.[Conclusion]Salmonella from H.asiaticum in grazing sheep in Emin County have certain drug resistance and pathogenic risks.The research results provide data support for the study of tick-borne pathogens.关键词
沙门菌/分离/鉴定/耐药性/毒力基因/蜱Key words
Salmonella/isolation/identification/drug resistance/virulence genes/ticks分类
农业科技引用本文复制引用
张旭,赫永强,郭慧琳,朱婷婷,唐于皓,李静,邓园萍,齐萌..额敏县放牧羊寄生蜱体内沙门菌的分离鉴定、耐药性与毒力分析[J].畜牧与饲料科学,2026,47(2):107-115,9.基金项目
新疆生产建设兵团第九师科技计划项目(2023JS002). （2023JS002）
