农药学学报2026，Vol.28Issue(3)：418-430,13.DOI:10.16801/j.issn.1008-7303.2026.0035
器官芯片与类器官芯片在环境污染物毒性评估中的应用与研究进展
Applications and research progress of organ-on-a-chip and organoid-on-a-chip in the toxicity assessment of environmental pollutants
摘要
Abstract
The growing health hazards posed by environmental pollutants have highlighted the urgent need to establish efficient and reliable toxicity assessment systems,which has become a key focus in toxicological research.Traditional toxicity evaluation primarily relies on animal testing and two-dimensional(2D)cell culture models.However,animal studies are limited by species-specific predictive biases and ethical controversies,while 2D cell models fail to accurately replicate human metabolic and toxicological responses due to their lack of three-dimensional microenvironments and intercellular interactions.To address these technical limitations,the development of novel in vitro evaluation models has become particularly crucial.In recent years,organ-on-a-chip technology has demonstrated unique advantages in toxicological research by precisely simulating the physiological microenvironment and functional characteristics of human organs.Notably,organoid-on-a-chip technology,which integrates three-dimensional organoid culture systems with microfluidic platforms,has further enhanced the biomimetic performance and assessment accuracy of these models.This approach shows broad application prospects in drug toxicity testing,disease mechanism research,and environmental pollutant risk assessment.This review provides a systematic overview of the research progress in the application of organ-on-a-chip and organoid-on-a-chip technologies for toxicity assessment of typical environmental pollutants,including pesticides,endocrine-disrupting chemicals,microplastics/nanoplastics,and antibiotics.It focuses on summarizing their applications and advantages in studies of multi-organ interactions,metabolic toxicity,and endocrine-disrupting mechanisms.Furthermore,the challenges and future development prospects of these technologies in environmental toxicology and health risk assessment are discussed,with the aim of offering new technical support for the scientific assessment and regulatory decision-making related to emerging environmental pollutants.关键词
器官芯片/类器官芯片/内分泌干扰作用/环境污染物/健康毒理学Key words
organ-on-a-chip/organoid-on-a-chip/endocrine disruption/environmental pollutants/health toxicology分类
生物科学引用本文复制引用
刘悦,许柠,邓慕宸,宋欣宇,孙然锋,王培龙,曹立冬..器官芯片与类器官芯片在环境污染物毒性评估中的应用与研究进展[J].农药学学报,2026,28(3):418-430,13.基金项目
农业科技重大项目. Supported by Agricultural Science and Technology Major Project. （）
