农业环境科学学报2026，Vol.45Issue(5)：1117-1128,12.DOI:10.11654/jaes.2025-0574
镉和苯甲羟肟酸复合污染对上海青生长、生理指标和细胞损伤的影响
Effects of co-exposure to cadmium and benzohydroxamic acid on growth,physiological parameters,and cellular damage in Brassica rapa shanghaiqing
摘要
Abstract
Based on the objective of investigating the compound pollution mechanisms of mineral processing agents and heavy metals in mining environments.This study employed a hydroponic experiment to examine the effects of combined cadmium(Cd)and benzohydroxamic acid(BHA)pollution on the growth,photosynthetic characteristics,and osmotic adjustment substance accumulation in Shanghaiqing.The localization of viable cells,dead cells,and H2O2 in plant roots was visualized using fluorescent probe techniques.The results demonstrated that pollution stress induced by Cd and BHA significantly inhibited the growth of Shanghaiqing.Compared with the control(CK)group,single Cd pollution,single BHA pollution,and Cd-BHA combined pollution reduced the shoot fresh weight by 44.15％,55.38％,and 63.29％,respectively,and the root fresh weight by 56.97％,67.24％,and 68.95％,respectively,indicating more severe toxicity under combined pollution.Under Cd and BHA stress,the net photosynthetic rate,transpiration rate,and stomatal conductance of Shanghaiqing exhibited trends consistent with fresh weight changes.The malondialdehyde(MDA)content in shoots under Cd-BHA combined pollution reached 6.0 times that of the CK group and was 2.6 times and 1.9 times higher than that in the single Cd and single BHA treatments,respectively.Furthermore,the accumulation of osmoregulatory substances-including proline,soluble sugar,and soluble protein in Shanghaiqing significantly exceeded that in the CK group.Staining with FDA,PI,and SYTOX™ Green revealed that root cell viability was further reduced under Cd-BHA combined stress compared with single stress conditions.The addition of BHA increased Cd content in the shoots by 10.7％,suggesting a synergistic effect of BHA on Cd uptake in plants.These findings indicate that combined Cd and BHA pollution exacerbates oxidative damage,disrupts osmotic regulation,and promotes root cell death through synergistic interactions,thereby intensifying toxic effects on Shanghaiqing and enhancing Cd accumulation in aboveground tissues.关键词
镉/苯甲羟肟酸/上海青/复合污染/光合作用/细胞毒性Key words
cadmium/benzohydroxamic acid/Brassica rapa shanghaiqing/compound pollution/photosynthesis/cytotoxicity分类
资源环境引用本文复制引用
邵婷婷,田丽燕,黄益宗,李世彪,赵冉,杨瑛斌,景睿,樊芳玲,黄晶心,李曦同..镉和苯甲羟肟酸复合污染对上海青生长、生理指标和细胞损伤的影响[J].农业环境科学学报,2026,45(5):1117-1128,12.基金项目
云南师范大学高层次人才引进项目(01700205020503144)Yunnan Normal University High-level Talent Introduction Project(01700205020503144) （01700205020503144）
