环境与职业医学2026，Vol.43Issue(5)：582-590,9.DOI:10.11836/JEOM25441
奶牛养殖工作场所肠球菌基因型多样性和抗生素耐药性
Genotypic diversity and antibiotic resistance of Enterococcus in dairy farming workplaces
摘要
Abstract
[Background]Under intensive dairy farming conditions,Enterococcus spp.can be transmitted between animals,farm workers,and the environment via multiple vectors such as feces,soil,water,air,and farming equipment,posing a potential threat to public health. [Objective]To elucidate the prevalence,distribution,and antimicrobial resistance profiles of En-terococcus faecalis(E.faecalis)and Enterococcus faecium(E.faecium)among farm workers,dairy cattle,and the farm environment in Xinjiang,and to assess the risk of their cross-host transmission. [Methods]From May 2024 to January 2025,a total of 317 samples were collected from 11 large-scale dairy farms in Xinjiang,China,including feces from farm workers(n=130)and dairy cattle(n=154),and environmental samples(n=33).E.faecalis and E.faecium were isolated and identi-fied,followed by antimicrobial susceptibility testing and multilocus sequence typing(MLST)to analyze their molecular characteristics. [Results]A total of 183 Enterococcus isolates were obtained(66 E.faecalis and 117 E.faecium isolated).The isolation rates of both species showed statistically significant differences among the three sources(χ2=29.21,P=0.003).Antimicrobial resistance analysis revealed that E.faecalis generally exhibited higher resistance rates across multiple antibiotic classes than E.faecium.High resistance to rifampicin was observed across all sources(50.00%-81.25%),with statistical variation among origins(χ2=8.03,P=0.024).Multidrug-resistant strains accounted for 69.10%of the isolates.Multidrug resistance patterns in E.faecium varied significantly by source(χ2=27.19,P=0.014),and one isolate displayed resistance to eight antibiotic classes.MLST indicated high genetic diversity;E.faecalis was dominated by ST472 and ST227 of which the distrubution was significantly different among sources,while E.faecium primarily clustered into clonal complexes CC94(centered on ST94)and CC17(centered on ST22). [Conclusion]Resistant Enterococcus strains exhibit cross-transmission among farm workers,animals,and the environment.Under the"One Health"framework,standardized farming protocols and prudent antimicrobial use are essential to disrupt the transmission chain of resistant clones and mitigate the spread of antimicrobial resistance at its source.关键词
粪肠球菌/屎肠球菌/抗生素耐药性/多位点序列分型Key words
Enterococcus faecalis/Enterococcus faecium/antimicrobial resistance/multilocus sequence typing分类
医药卫生引用本文复制引用
卫香楠,胥婉婷,吴建勇,李富业,陈阳贵,黄佳,王福龙,金纪国,武凡,马熙骁,王召杰,王星宇..奶牛养殖工作场所肠球菌基因型多样性和抗生素耐药性[J].环境与职业医学,2026,43(5):582-590,9.基金项目
新疆维吾尔自治区自然科学基金重点项目(2023D01D06)新疆维吾尔自治区第三批"天山英才"青年拔尖人才-青年科技创新人才项目(2024TSYCCX0083) （2023D01D06）
自治区研究生科研创新项目(XJ2025G152) （XJ2025G152）
