辽宁中医杂志2026，Vol.53Issue(6)：211-218,后插9,9.DOI:10.13192/j.issn.1000-1719.2026.06.051
补肾活血导法调控围着床期LXA4R/NF-κB及上皮-间质转化时序表达改善大鼠胚胎植入的机制研究
Study on Mechanism of Bushen Huoxue Recipe(补肾活血方)Enema in Improving Embryo Implantation by Regulating LXA4R/NF-κB and Epithelial-Mesenchymal Transition Sequential Expressions in Rats During Peri-Implantation Period
摘要
Abstract
Objective To study the mechanism of Bushen Huoxue Recipe(补肾活血方,BHR)enema in improving embryo im-plantation by regulating lipoxin A4 receptor(LXA4R)/nuclear factor kappa-B(NF-κB)and epithelial-mesenchymal transi-tion(EMT)sequential expressions in the rats during the peri-implantation period,and draw the sequential expression profiles.Methods The rat model of kidney deficiency and blood stasis-implantation disorder was replicated by hydroxyurea and adrenali-necombined with LXA4.SD rats were randomly divided into the blank group,the model group,and the traditional Chinese medi-cine(TCM)group.The rats in each group were sampled on the 4th day post coition(4 dpc),6 dpc and 8 dpc.Then the general condition and the number of embryo implantations were observed.The four items of coagulation were detected.The endometrial morphology and pinopode development were observed by HE staining and scanning electron microscope(SEM),respectively.Be-sides,ELISA was used to test the levels of serum estradiol(E2)and progesterone(P).Western blot and RT-qPCR were applied to detect the protein and mRNA expressions of LXA4R,(p-)NF-κB p65,E-cadherin and N-cadherin in the uterus.Results BHR enema could improve the general condition of the model rats,significantly raise the uterine index and embryo implantation number of 8 dpc model rats(P＜0.05),increase the plasma thrombin time(TT)of 4/6 dpc model rats(P＜0.05),reduce the plasma fibrinogen(FIB)content(P＜0.05),and improve the endometrium morphology and pinocytosis process development in the peri-implantation period of model rats.BHR enema could also distinctly down-regulate the expressions of LXA4R protein and mRNA in the uterus of model rats on 6 dpc(P＜0.05),as well as E-cadherin protein,and markedly up-regulate the levels of NF-κB p65 phosphorylated protein and mRNA,as well as the levels of N-cadherin protein and mRNA(P＜0.05).Conclu-sion BHR enema can improve the syndrome of model rats,promote the synchronous development of the endometrium and embryo in model rats,significantly regulate the sequential expressions of LXA4R/NF-κB and EMT in the uterus of peri-implantation model rats,and repair the homeostasis of the"anti-inflammatory-pro-inflammatory-anti-inflammatory"microenvironment and EMT processes at the maternal-fetal interface to a certain extent,thus improvingembryo implantation.关键词
补肾活血导法/胚胎植入/脂氧素A4受体/核因子-κB/上皮-间质转化/时序表达Key words
Bushen Huoxue Recipe(补肾活血方)enema/Lipoxin A4 receptor/nuclear factor kappa-B/epithelial-mesen-chymal transition/embryo implantation/sequential expression分类
医药卫生引用本文复制引用
朱璨,夏宛廷,周航,张璇,王毅,黄金珠,曾倩..补肾活血导法调控围着床期LXA4R/NF-κB及上皮-间质转化时序表达改善大鼠胚胎植入的机制研究[J].辽宁中医杂志,2026,53(6):211-218,后插9,9.基金项目
国家自然科学基金面上项目(82174430) （82174430）
