农药学学报2026，Vol.28Issue(3)：473-486,14.DOI:10.16801/j.issn.1008-7303.2026.0036
银杏酸对立枯丝核菌的抑制作用及其转录组分析
Inhibitory effect of ginkgolic acids on Rhizoctonia solani and transcriptome analysis
摘要
Abstract
This study aimed to investigate the antifungal activity of ginkgolic acids(GAs)against Rhizoctonia solani and preliminarily explore their underlying action mechanisms.The inhibitory effect of GAs on fungal growth was determined using the mycelial growth rate method.Scanning electron microscopy(SEM)was used to obverse morphological change of mycelia after GAs treatment.Additionally,the cell membrane permeability,malondialdehyde(MDA),and soluble protein content of GAs-treated mycelia were measured,along with the activities of catalase(CAT),superoxide dismutase(SOD),malate dehydrogenase(MDH),and succinate dehydrogenase(SDH).Transcriptome sequencing was employed to identify differentially expressed genes and conduct pathway enrichment analysis.Moreover,reverse transcription real-time quantitative PCR(RT-qPCR)was applied to verify the expression levels of key DEGs.The results showed that GAs exhibited significant antifungal activity against R.solani,with an EC50 of 0.234 mg/mL.At a concentration of 0.300 mg/mL,the inhibition rate reached 60.59％,and the mycelia showed abnormal morphology,including shriveling,collapse,and disruption of branching structures.After 4 h of treatment with 0.300 mg/mL GAs,mycelium cell membrane permeability and MDA content significantly increased,while CAT,SOD,MDH,and SDH activities significantly decreased.Transcriptome analysis revealed that GAs induced 2018 DEGs,consisting of 906 upregulated and 1112 downregulated genes.These DEGs were significantly enriched in metabolic pathways,including fatty acid degradation,peroxisome,and steroid biosynthesis.RT-qPCR validation results are highly consistent with the transcriptome data.This study provides theoretical basis for the development and application of GAs as plant-derived antifungal agents.关键词
银杏酸/立枯丝核菌/抑菌活性/生理生化指标/基因/转录组测序Key words
ginkgolic acids/Rhizoctonia solani/antifungal activity/physiological and biochemical indices/gene/transcriptome sequencing分类
农业科技引用本文复制引用
殷文闻,宋江鱼,罗紫琳,何珺,彭丽娟..银杏酸对立枯丝核菌的抑制作用及其转录组分析[J].农药学学报,2026,28(3):473-486,14.基金项目
银杏酸微乳剂研制及植物炭疽病防治研究(黔科合基础-ZK[2022]一般094) （黔科合基础-ZK[2022]一般094）
贵州省烟草提质增效全省重点实验室(黔科合平台ZSYS[2025]028). Supported by Supported by the Development of Ginkgolic Acids Micromulsion and Their Application in Controlling Anthracnose in Plants(No.QKHJC-ZK[2022]YB094) （黔科合平台ZSYS[2025]028）
the Guizhou Provincial Key Laboratory for Tobacco Quality Improvement and Efficiency Enhancement(Qiankehe platform ZSYS[2025]028). （Qiankehe platform ZSYS[2025]028）
