农业环境科学学报2026，Vol.45Issue(5)：1179-1190,12.DOI:10.11654/jaes.2025-0444
稻虾共作对稻田土壤微生物群落结构和功能特征的影响
Effects of rice and shrimp on soil microbial community structure and functional characteristics in paddy field
摘要
Abstract
To elucidate the impact of the rice-crayfish co-culture system(DX)on soil microbial community structure and function,a metagenomic sequencing approach was employed to analyze the structural and functional diversity of the soil microbiota during the rice maturity stage,using traditional rice monoculture(CK)as the control.The results demonstrated that the DX system significantly enhanced soil microbial alpha diversity compared to CK(P＜0.05).The dominant phyla in both systems were Pseudomonadota(25.34％-31.22％),Planctomycetota(10.39％-14.66％),Actinomycetota(10.84％-13.69％),and Chloroflexota(8.69％-11.48％),though their relative abundances varied.Notably,DX significantly increased the abundance of Pseudomonadota and Thermodesulfobacteriota(P＜0.05).At the genus level,Singulisphaera(5.37％-7.99％),Bradyrhizobium(4.71％-5.35％),Ktedonobacter(2.30％-5.09％),and Pseudolabrys(2.73％-3.77％)were dominant in both soils.Compared with CK,DX significantly elevated the abundances of Bradyrhizobium,Pseudolabrys,Anaerolinea,and Methyloceanibacter.Correlation analysis indicated that soil pH and available potassium(AK)were significantly correlated with the relative abundance of major microbial taxa.KEGG(Kyoto Encyclopedia of Genes and Genomes)functional annotation revealed that metabolism was the predominant function of farmland soil microorganisms,accounting for 50.5％-51.23％of annotated functions.Compared to CK,DX increased the proportions of functional genes involved in carbon metabolism,two-component system,quorum sensing,and ABC transporters.Functional enrichment analysis further showed that the DX system enriched more pathways associated with metabolism,biosynthesis,and transport.COG(Clusters of Orthologous Groups)annotation confirmed that DX had significantly higher relative abundance in 8 out of the top 10 functional categories,5 of which were related to cellular transport and metabolism.In conclusion,the rice-crayfish co-culture system significantly enhances soil microbial diversity,enriches functional gene repertoires,and strengthens the metabolic and transport potential of the soil microbial community.关键词
稻虾共作/微生物/宏基因组测序/群落结构/功能基因Key words
rice-crayfish co-culture/microorganism/metagenomic sequencing/community structure/functional gene分类
农业科技引用本文复制引用
杜春朝,倪才英,刘丽,蒋志忠..稻虾共作对稻田土壤微生物群落结构和功能特征的影响[J].农业环境科学学报,2026,45(5):1179-1190,12.基金项目
国家自然科学基金项目(42167006) （42167006）
江西省现代农业产业技术体系建设专项项目(JXARS-12-环境控制) （JXARS-12-环境控制）
江西省教育厅科学技术项目(GJJ210328)National Natural Science Foundation of China(42167006) （GJJ210328）
Jiangxi Province Modern Agricultural Industrial Technology System Construction Project(JXARS-12-Environmental Control) （JXARS-12-Environmental Control）
Science and Technology Project of Jiangxi Provincial Department of Education(GJJ210328) （GJJ210328）
