农药学学报2026，Vol.28Issue(3)：516-523,8.DOI:10.16801/j.issn.1008-7303.2026.0034
焦曲霉SN56147次生代谢产物分离鉴定及其对灰葡萄孢的抑制作用
Isolation and identification of secondary metabolites from Aspergillus ustus SN56147 and their inhibitory effects on Botrytis cinerea
摘要
Abstract
Silica gel column chromatography,gel column chromatography and high-performance liquid chromatography were used to isolate and purify secondary metabolites from Aspergillus ustus SN56147.The structures of ten compounds were identified via high-resolution mass spectrometry(HRMS),1H NMR,13C NMR,2D NMR and literature data comparison.Compound 1(Q-methylalbrassitriol)was characterized as a new sesquiterpenoid,whereas compounds 2-10 were known substances.The mycelial growth rate method was employed to test the antifungal activity against Botrytis cinerea,and wound inoculation and detached leaf assays were conducted to assess the control efficacy of active compounds on strawberry gray mold.The results revealed that compound 3(6-ethyl-2,4-dihydroxy-3-methylbenzaldehyde)exerted strong inhibitory activity against B.cinerea with an EC50 value of 27.14 μg/mL.At 400 μg/mL,its protective and curative effects on strawberry were 51.85％and 59.26％respectively,close to those of the positive control pyrimethanil,suggesting its promising prospect for further investigation.关键词
焦曲霉/次生代谢产物/分离鉴定/灰葡萄孢/抑菌活性/草莓灰霉病Key words
Aspergillus ustus/secondary metabolites/isolation and identification/Botrytis cinerea/antifungal activity/strawberry gray mold disease分类
化学化工引用本文复制引用
王梓源,于雅伦,邓芳勤,于志国..焦曲霉SN56147次生代谢产物分离鉴定及其对灰葡萄孢的抑制作用[J].农药学学报,2026,28(3):516-523,8.基金项目
国家重点研发计划(No.2018YFD0800401). Supported by National Key R&D Program of China((No.2018YFD0800401). （No.2018YFD0800401）
