蔬菜Issue(6)：45-52,8.DOI:10.26924/j.cnki.sczz.2026.6006
韭菜白腐病病原菌的分离与鉴定
Isolation and Identification of the Pathogen Causing White Rot Disease of Chinese Chive
周倩 1王玉 1席海龙 1隋书婷 1翟婷婷 1葛家成 1张宗俭1
作者信息
- 1. 海利尔药业集团股份有限公司,山东青岛 266109
- 折叠
摘要
Abstract
To identify the pathogen species causing sclerotinia disease in greenhouse chives in Lintao county,Dingxi city,Gansu province,and to provide an etiological basis for local disease control,this study collected typical diseased Chinese chive samples from the local area.A strain designated 25-4-GSJC was isolated and purified.Pathogenicity was confirmed through in vitro leaf inoculation,in vivo potted soil inoculation,and artificial re-inoculation.The pathogen was identified using morphological observation,ITS sequence PCR amplification and sequencing,and phylogenetic analysis.The results showed that both wound inoculation and potted soil inoculation induced typical field disease symptoms on Chinese chives.The re-isolated strain from inoculated plants exhibited the same colony morphology as the original strain,satisfying Koch's postulates.On PDA medium,the mycelium was white,no conidia were produced,and small,dense black sclerotia were formed.An 814 bp ITS gene fragment was amplified by PCR.Sequence homology and phylogenetic tree analysis indicated that the tested strain clustered with the type strain of Stromatinia cepivora in the same evolutionary clade with 96％bootstrap support.In conclusion,the pathogen causing sclerotinia disease in greenhouse Chinese chives in this region is firstly identified as Stromatinia cepivora.关键词
韭菜/病害/病原菌/分离/鉴定/白腐小核菌/菌核/白腐病Key words
Chinese chive/disease/pathogen/isolation/identification/Stromatinia cepivora/sclerotia/white rot分类
农业科技引用本文复制引用
周倩,王玉,席海龙,隋书婷,翟婷婷,葛家成,张宗俭..韭菜白腐病病原菌的分离与鉴定[J].蔬菜,2026,(6):45-52,8.
