农药学学报2026，Vol.28Issue(3)：563-573,11.DOI:10.16801/j.issn.1008-7303.2026.0032
促生-降解菌群对复合农药污染修复及对蔬菜根际微生态的影响
Remediation of combined pesticide contamination by plant growth-promoting and degrading consortia and their effects on vegetable rhizosphere microecology
摘要
Abstract
To investigate the efficacy of multifunctional microbial consortia in remediating combined pesticide contamination in the vegetable-soil system and their impact on the rhizosphere microecology of vegetables,this study used bacterial strains isolated and screened from the rhizosphere of pak choi cultivated in pesticide-contaminated fields.Four functional microbial consortia were constructed:plant growth-promoting(T1),pesticide-degrading(T2),dual-functional growth-promoting and degrading(T3),and multifunctional integrated(T4).Through pot experiments,the remediation effect of different consortia on combined pesticide contamination in the soil-pak choi system and their influence on plant quality were systematically evaluated,and high-throughput sequencing was employed to elucidate the underlying rhizosphere microecological mechanisms.The results demonstrated that:1)The T3 consortium achieved the highest pesticide degradation efficiency in both soil and pak choi,with an average increase of 44.3％and 49.1％,respectively,compared to the control(CK),demonstrating significantly superior performance compared to the other consortia(P<0.05);2)T3 treatment significantly promoted pak choi growth(biomass increased by 76.5％),improved nutritional quality(with increases of 15.4％,17.1％,and 15.2％in vitamin C,soluble sugar,and total protein content,respectively),and enhanced soil fertility;3)Rhizosphere microbiome analysis revealed that T3 treatment specifically enriched the genera Burkholderia and Massilia.Their relative abundances increased approximately threefold and twofold compared to the T1 and T2 groups,respectively,exhibiting significant positive correlation with pesticide degradation rates.The findings indicate that the growth-promoting and pesticide-degrading microbial consortium(T3)achieves both efficient pesticide degradation and crop quality improvement through the synergistic modulation of rhizosphere functional microbial communities.This study provides a theoretical basis and a promising strategy for the in situ remediation of combined pesticide contamination in agricultural fields.关键词
复合菌剂/农药污染/协同修复/蔬菜品质/微生态机制Key words
composite microbial inoculant/pesticide pollution/collaborative remediation/improvement of vegetable quality/micro-ecological mechanism分类
农业科技引用本文复制引用
毛甜甜,董成,陈静,李梅,马丽雅,李勇,余向阳,葛静..促生-降解菌群对复合农药污染修复及对蔬菜根际微生态的影响[J].农药学学报,2026,28(3):563-573,11.基金项目
国家重点研发项目(No.2023YFD1400903). Supported by The National Key Research and Development Program of China(No.2023YFD1400903). （No.2023YFD1400903）
