热带作物学报2026，Vol.47Issue(6)：1389-1397,9.DOI:10.3969/j.issn.1000-2561.2026.06.003
基于转录组解析斯里兰卡木薯花叶病毒侵染对木薯叶片水杨酸合成的调控作用
Transcriptome-based Analysis of the Regulatory Role of Sri Lankan Cassava Mosaic Virus Infection in Salicylic Acid Biosynthesis in Cas-sava Leaves
摘要
Abstract
Cassava is an important tropical food and cash crop,but the production is severely threatened by Sri Lankan cassava mosaic virus(SLCMV).In this study,healthy and SLCMV-infected cassava cultivar NZ199 were used as mate-rials to systematically analyze the impact of SLCMV infection on gene transcriptional activity in cassava leaves.Tran-scriptome data analysis identified a total of 4728 differentially expressed genes.Compared with healthy leaves,4495 genes were up-regulated and 233 genes were down-regulated in infected leaves.Further analysis revealed that in in-fected leaves,the expression of key genes in the isochorismate synthase(ICS)pathway of salicylic acid biosynthesis was suppressed,while key genes in the phenylalanine ammonia-lyase(PAL)pathway were activated.Liquid chroma-tography-mass spectrometry(LC-MS)detection showed that,compared to healthy leaves,salicylic acid content in in-fected leaves increased by 51.98%,while the content of salicylic acid glucoside decreased by 10.95%.In conclusion,SLCMV infection inhibited the expression of genes such as ICS and PBS3 in the ICS pathway,while activating genes such as PAL,CNL and BEBT in the PAL pathway,ultimately leading to increased salicylic acid accumulation in cassava leaves.This study would provide a theoretical basis for understanding the molecular mechanisms of cassava's response to SLCMV and for breeding disease-resistant varieties.关键词
木薯/斯里兰卡木薯花叶病毒/水杨酸/转录组Key words
cassava/Sri Lankan cassava mosaic virus(SLCMV)/salicylic acid/transcriptome分类
农业科技引用本文复制引用
杨浩杰,李亚军,李远超,刘晓晨,陈新,曾长英..基于转录组解析斯里兰卡木薯花叶病毒侵染对木薯叶片水杨酸合成的调控作用[J].热带作物学报,2026,47(6):1389-1397,9.基金项目
热带作物生物育种全国重点实验室创新团队科研项目(No.NKLTCBCXTD07) （No.NKLTCBCXTD07）
财政部和农业农村部国家现代农业产业技术体系建设专项(No.CARS-11-HNCX). （No.CARS-11-HNCX）
