农业生物技术学报2026，Vol.34Issue(7)：1383-1398,16.DOI:10.3969/j.issn.1674-7968.2026.07.002
低温胁迫下白菜型冬油菜BrSAMS2蛋白的鉴定与分析
Identification and Analysis of BrSAMS2 Protein in Winter Brassica rapa Under Low Temperature Stress
摘要
Abstract
Winter Brassica rapa is an important oilseed crop in China,with strong adaptability,excellent agronomic traits and high production efficiency.Low-temperature adversity ranks among the most critical abiotic constraints throughout its developmental stages.To understand the molecular mechanism of cold resistance of winter B.rapa,in this study,two-dimensional electrophoresis was employed to identify differentially expressed proteins in the shoot apical meristem of winter B.rapa'Longyou 7'exposed to low-temperature stress.Furthermore,bioinformatics approaches were utilized to perform functional annotation and pathway enrichment analysis on these differentially expressed proteins.The results showed that low-temperature stress induced 24 significantly differentially expressed proteins,among which 8 were up-regulated,10 were down-regulated,and 6 were induced expression proteins.Go annotation results revealed that the majority of differentially expressed proteins participated in the response to low-temperature stress,antioxidant defense,energy metabolism,and cell structure maintenance processes.Analysis based on the KEGG database uncovered the pivotal roles played by differentially expressed proteins in carbon metabolism,photosynthetic carbon fixation,as well as multiple amino acid biosynthetic pathways.S-adenosylmethionine synthase 2(SAMS2)gene was associated with low-temperature response and had the highest expression level at 12 h of low-temperature treatment.The encoded protein was a stable hydrophilic protein with a conserved domain,and its promoter region contained cis-acting elements such as low-temperature response.BrSAMS2 might participate in the low-temperature response by regulating methyl donors and ethylene synthesis.This study provides new candidate genes and protein targets for revealing the molecular mechanism of cold tolerance in winter rapeseed.关键词
白菜型冬油菜/低温胁迫/双向电泳/差异蛋白/抗寒性Key words
Winter Brassica rapa/Low-temperature stress/Two-dimensional electrophoresis/Differentially expressed proteins/Cold resistance分类
农业科技引用本文复制引用
李博文,马骊,陶肖蕾,张媛媛,武军艳,王一帆,孙浩,李诗艺,徐正南,蔡芊依..低温胁迫下白菜型冬油菜BrSAMS2蛋白的鉴定与分析[J].农业生物技术学报,2026,34(7):1383-1398,16.基金项目
甘肃省联合科研基金一般项目(24JRRA837) （24JRRA837）
国家自然科学基金(32260519) （32260519）
甘肃农业大学伏羲人才项目(Gaufx-05Y01) （Gaufx-05Y01）
兰州市青年科技人才创新项目(2024-QN-45) （2024-QN-45）
甘肃省高校青年博士支持项目(2024QB-065) （2024QB-065）
甘肃省科技重大专项(24ZDNA007) （24ZDNA007）
财政部和农业农村部国家现代农业产业技术体系(CARS-12-09) （CARS-12-09）
