热带作物学报2026，Vol.47Issue(6)：1398-1412,15.DOI:10.3969/j.issn.1000-2561.2026.06.004
辣椒CaPC家族成员的全基因组鉴定及花药特异基因的挖掘
Genome-wide Identification of the Pepper CaPC Gene Family and Discovery of the Anther-specific Genes
摘要
Abstract
Phytocyanin(PC)is a class of plant-specific type I copper-binding proteins that play crucial roles in plant growth and development.To date,genome-wide identification of the PC gene family and its function in pollen devel-opment remain unreported in pepper.In this study,based on the genomic and transcriptomic data of pepper(Capsicum annuum L.),we conducted systematic identification and preliminary functional characterization of the PC gene family(CaPC)in pepper by integrating genomic,bioinformatic,and molecular biological approaches.The results revealed that the pepper genome contained a total of 45 CaPC genes,which were unevenly distributed across 11 chromosomes with partial clustering.Phylogenetic analysis indicated that CaPCs could be classified into three subfamilies,UCL,SCL and ENODL,comprising 6,8 and 31 members,respectively.Gene structure and conserved domain analyses showed that most CaPC genes contained two exons,and all CaPC members possessed the conserved Cu_bind-like domain.Whole-genome synteny analysis revealed that 11 pairs of CaPC genes were generated by segmental duplication in the pepper genome.Synteny analysis with closely related species indicated that the number of syntenic PC gene pairs was highly conserved among pepper,tomato,and eggplant.Promoter cis-acting element analysis showed that the promoter regions of CaPC genes harbored multiple regulatory elements associated with light responsiveness,hormone response,stress response,and growth and development.Expression profiling analysis identified four CaPC genes specifically expressed in anthers.Among them,CaENODL10.1 exhibited specific expression in anthers at developmental stages 6-9.Further RT-PCR analysis revealed that CaENODL10.1 was not expressed in anthers of either cytoplasmic-nuclear inte-raction male sterility(CMS)or genetic male sterility(GMS)pepper lines,suggesting that this gene may serve as a key factor in pollen development.In conclusion,this study conducted a systematic analysis of the CaPC family genes in pepper.The findings would provide novel insights into the role of PC genes in plant pollen development and offer po-tential molecular targets for the development of male sterile lines in pepper.关键词
辣椒/蓝铜蛋白/花药特异基因/雄性不育Key words
pepper/phytocyanin/anther-specific gene/male sterility分类
农业科技引用本文复制引用
吴明华,董骥驰,赵晓圆,陈姝晓,程蛟文,胡芳,王俊,李琼,莫昊天,化青珠..辣椒CaPC家族成员的全基因组鉴定及花药特异基因的挖掘[J].热带作物学报,2026,47(6):1398-1412,15.基金项目
广州市科技计划项目(No.2025A04J4138) （No.2025A04J4138）
国家自然科学基金项目(No.32302562) （No.32302562）
广东省自然科学基金项目(No.2024A1515013247). （No.2024A1515013247）
