农药学学报2026，Vol.28Issue(3)：607-612,6.DOI:10.16801/j.issn.1008-7303.2026.0050
冰城链霉菌HU176中两个米尔贝霉素D异构体的分离鉴定及其杀线虫活性
Isolation,identification,and nematicidal activity of two milbemycin D isomers from Streptomyces bingchenggensis HU176
摘要
Abstract
To discover novel milbemycin-type compounds with insecticidal activity,two isomers(1 and 2)of milbemycin D with nematicidal activity were isolated and purified from the secondary metabolites of Streptomyces bingchenggensis HU176.Purification was achieved using gel column chromatography and high-performance liquid chromatography(HPLC).Based on comprehensive spectroscopic analyses(1H NMR,13C NMR,HRESI-MS)and comparison with literature data,their structures were identified as 12-desmethyl-12-ethyl milbemycin A4 and 24-desmethyl-24-ethyl milbemycin A4,respectively.Bioassay results showed that compounds 1 and 2 exhibited moderate nematicidal activity against Bursaphelenchus xylophilus,with LC50 values of 14.53 and 16.89 μg/mL,respectively.These values were comparable to that of the positive control avermectin B1a(LC50=12.42 μg/mL).Notably,compound 1 is a new milbemycin analog,and compound 2,a known compound,is reported as a natural product for the first time.These findings provide a valuable reference for the further development and utilization of milbemycin-type compounds.关键词
冰城链霉菌HU176/米尔贝霉素D/异构体/杀线虫活性/松材线虫Key words
Streptomyces bingchenggensis HU176/milbemycin D/isomers/nematicidal activity/Bursaphelenchus xylophilus分类
农业科技引用本文复制引用
贾雪莹,杨可,徐海菊,王继栋,张绍勇,张辉,郑晓鹤,张继..冰城链霉菌HU176中两个米尔贝霉素D异构体的分离鉴定及其杀线虫活性[J].农药学学报,2026,28(3):607-612,6.基金项目
台州市科技计划(No.24nyb10). Supported by Taizhou Science and Technology Plan Project(No.24nyb10). （No.24nyb10）
