南京中医药大学学报2026，Vol.42Issue(6)：831-841,11.DOI:10.14148/j.issn.1672-0482.2026.0831
基于非靶向代谢组学技术研究蛹虫草核苷的抗肺癌机制
Investigation on the Anti-Lung Cancer Mechanism of Cordyceps Militaris Nucleosides by Non-Targeted Metabolomics
摘要
Abstract
OBJECTIVE To screen the active fraction of Cordyceps militaris(CM)exerting anti-lung cancer effects,investigate its effects on endogenous metabolites in lung cancer-bearing nude mice and explore the potential mechanism of action by non-targeted metabolomics.METHODS The nucleosides(CMN)and polysaccharides(CMP)of CM were extracted and purified.The components in CMN were identified by UPLC-LTQ-Orbitrap X instrument.The model of human lung cancer xenograft in nude mice was estab-lished,and treated with CM and its different fraction extracts for two weeks.The active fraction of CM with anti-lung cancer effect was screened by monitoring tumor volume,tumor weight and histopathological alterations.Serum and tumor tissue samples were collected,and GC-MS technology was used for metabolomics analysis.The effects of CMN on lung cancer cells were evaluated by CCK-8,colony formation,wound healing assays and cell cycle analysis.The metabolic pathways involved in the anti-lung cancer effect of CMN were further verified by cellular metabolomics.RESULTS A total of 13 components including cordycepin,inosine and adenine were identi-fied from CMN.CMN significantly inhibited tumor growth with low toxicity.At the cellular level,CMN inhibited the proliferation,mi-gration and cell cycle progression of lung cancer cells.A total of 38 differential metabolites were identified by serum metabolomics analyses,and 44 by tumor tissue metabolomics analyses,which were mainly involved in the citrate cycle(TCA cycle).This conclusion was further confirmed by differential metabolites identified through tumor cell metabolomics analyses.CONCLUSION CMN is the active fraction of CM exerting anti-lung cancer effects,which might exert anti-lung cancer effects by regulating the TCA cycle pathway.关键词
蛹虫草/肺癌/三羧酸循环/非靶向代谢组学/核苷Key words
Cordyceps militaris/lung cancer/TCA cycle/non-targeted metabolomics/nucleosides分类
医药卫生引用本文复制引用
柴晓倩,陆颖颖,陈雨雨,罗子宸,单进军,张雯,狄留庆..基于非靶向代谢组学技术研究蛹虫草核苷的抗肺癌机制[J].南京中医药大学学报,2026,42(6):831-841,11.基金项目
江苏省高等学校自然科学研究项目(19KJB360004) （19KJB360004）
江苏省研究生科研与实践创新计划项目(KYCX24_2297) （KYCX24_2297）
