南京中医药大学学报2026，Vol.42Issue(6)：842-854,13.DOI:10.14148/j.issn.1672-0482.2026.0842
刮痧干预对帕金森病模型大鼠运动障碍及氧化应激水平的影响
Gua Sha Intervention on Motor Dysfunction and Oxidative Stress Levels in a Rat Model of Parkinson's Disease
摘要
Abstract
OBJECTIVE To observe the effects of Gua Sha on motor dysfunction and oxidative stress levels in rotenone-induced Parkinson's disease(PD)model rats.METHODS Forty-eight SD rats were randomly divided into the blank group,sham-operated group,model group,drug group,sham Gua Sha group,and Gua Sha group,with 8 rats in each group.A PD rat model was established by stereotactic injection of 1 μL rotenone solution(12 μg·μL-1)into the left substantia nigra.The blank group,sham-operated group,and model group received no intervention.The drug group,sham Gua Sha group,and Gua Sha group received corresponding interventions from d14 after modeling.The drug group was given selegiline hydrochloride suspension at 0.5 mg·kg-1 by gavage for 23 consecutive days.The Gua Sha group received Gua Sha intervention,once every other day for a total of 12 sessions,ending on d36 after modeling.The sham Gua Sha group was only subjected to the same duration of handling and fixation as the Gua Sha group,without any other inter-vention.After intervention,open field test(OFT)and gait analysis were performed in each group.Immunofluorescence(IF)was used to detect tyrosine hydroxylase(TH)fluorescence intensity in the substantia nigra.Western blot was used to detect the protein expression of TH,α-Syn,Keap1,and Nrf2 in the substantia nigra.qRT-PCR was used to detect the mRNA expression of TH,α-Syn,Keap1,Nrf2,HO-1,and NQO1 in the substantia nigra.ELISA was used to detect striatal ROS and serum MDA,SOD,and GSH-Px levels.RESULTS Compared with the sham-operated group,the model group showed a significantly shortened total distance in the OFT(P<0.001),significantly reduced time spent in the central zone(P<0.01),and significantly fewer entries into the central zone(P<0.001).The gait cycle was prolonged,while the mean speed(P<0.01),paw area,and stride length were decreased.TH fluorescence intensity in the substantia nigra was decreased(P<0.01),TH protein and mRNA expression were decreased(P<0.001),and α-Syn protein and mRNA expression were increased(P<0.001).Striatal ROS was increased(P<0.05),serum MDA was increased(P<0.01),and SOD and GSH-Px were decreased(P<0.001).GSH and GSH-Px in the substantia nigra were decreased(P<0.01).Keap1 protein expres-sion in the substantia nigra was increased,while Nrf2 protein expression was decreased(P<0.001).The mRNA expression of Nrf2,HO-1,and NQO1 in the substantia nigra was decreased(P<0.05,P<0.01,P<0.001),Keap1 mRNA expression was increased(P<0.001),and G6PD and PGD protein expression in the substantia nigra was decreased(P<0.05,P<0.001).Compared with the model group,the Gua Sha group showed an increased total distance in the OFT(P<0.01),increased time spent in the central zone(P<0.01),and increased entries into the central zone.The gait cycle was shortened,while the mean speed(P<0.05),paw area,and stride length were increased.TH fluorescence intensity in the substantia nigra was enhanced(P<0.01),TH protein and mRNA expression were in-creased(P<0.01,P<0.001),and α-Syn protein and mRNA expression were decreased(P<0.01,P<0.001).Striatal ROS decreased,serum MDA decreased,serum SOD and GSH-Px increased,and substantia nigra GSH and GSH-Px increased(P<0.05).Keap1 pro-tein expression in the substantia nigra was decreased,while Nrf2 protein expression was increased(P<0.001).The mRNA expression of Nrf2,HO-1,and NQO1 in the substantia nigra was increased(P<0.05,P<0.01,P<0.001),while Keap1 mRNA expression was de-creased(P<0.01).G6PD and PGD protein expression in the substantia nigra was increased.The therapeutic effect of the Gua Sha group was comparable to that of the drug group.CONCLUSION Gua Sha can improve spontaneous activity and gait dysfunction in PD model rats,and its mechanism may be related to activation of the Keap1/Nrf2 oxidative stress pathway,reduction of oxidative dam-age,and protection of dopaminergic neurons.关键词
帕金森病/氧化应激/鱼藤酮/刮痧/Keap1/Nrf2/黑质/THKey words
Parkinson's disease/oxidative stress/rotenone/Gua Sha/Keap1/Nrf2/substantia nigra/TH分类
医药卫生引用本文复制引用
张馨悦,王庆,徐桂华,王秋琴,翁恒,朱颖,沙春颖,梁进浩,施慧,丁椤茹,刘洁..刮痧干预对帕金森病模型大鼠运动障碍及氧化应激水平的影响[J].南京中医药大学学报,2026,42(6):842-854,13.基金项目
国家自然科学基金青年科学基金项目(82405604) （82405604）
江苏省中医药科技发展计划面上项目(MS2024007) （MS2024007）
江苏省研究生科研创新计划项目(KYCX25_2384) （KYCX25_2384）
