南京中医药大学学报2026，Vol.42Issue(6)：870-881,12.DOI:10.14148/j.issn.1672-0482.2026.0870
石菖蒲挥发油活性成分甲基丁香酚靶向JAK2/STAT3通路缓解脑微血管内皮细胞炎症和焦亡
Methyl eugenol,an Active Component of Acorus Tatarinowii Volatile Oil,Targets the JAK2/STAT3 Path-way to Alleviate Inflammation and Pyroptosis in Brain Microvascular Endothelial Cells
摘要
Abstract
OBJECTIVE To investigate the protective effects and potential mechanisms of Acorus tatarinowii volatile oil and its main components(α-asarone,β-asarone,and methyl eugenol)on inflammation and pyroptosis in lipopolysaccharide(LPS)-induced brain microvascular endothelial cells(bEnd.3).METHODS An in vitro inflammation model was established using LPS-stimulated bEnd.3 cells.The LPS-stimulated bEnd.3 cells were then treated with Acorus tatarinowii volatile oil and its main active components(α-asarone,β-asarone,and methyl eugenol)to investigate their effects on inflammation and pyroptosis.Network pharmacology and mo-lecular docking analysis were used to identify potential targets of methyl eugenol.The STAT3 inhibitor Stattic was used to further vali-date the role of the signaling pathway.RESULTS The volatile oil of Acorus tatarinowii and its main active ingredient,methyl euge-nol,both exhibited significant anti-inflammatory and anti-pyroptosis effects,effectively inhibiting inflammation and pyroptosis.Net-work pharmacology analysis suggested that the JAK2/STAT3 signaling pathway might be a potential target of methyl eugenol,and mo-lecular docking experiments further confirmed that methyl eugenol had a strong binding affinity for both JAK2 and STAT3 proteins.In the LPS-induced bEnd.3 cell model,both Acorus tatarinowii volatile oil and methyl eugenol significantly reduced the phosphorylation level of STAT3;simultaneously,the STAT3-specific inhibitor Stattic also inhibited the expression of the pyroptosis marker protein cleaved caspase-1,further validating the key role of this pathway in regulating pyroptosis.CONCLUSION Methyl eugenol can sig-nificantly inhibit LPS-induced pyroptosis and inflammatory responses in bEnd.3 cells by targeting the JAK2/STAT3 signaling path-way.The findings offer a new strategy for the prevention and treatment of neuroinflammation-related diseases.关键词
石菖蒲挥发油/炎症/细胞焦亡/JAK2/STAT3信号通路Key words
Acorus tatarinowii volatile oil/inflammation/pyroptosis/JAK2/STAT3 signaling pathway分类
医药卫生引用本文复制引用
谭越雯,陈娟娟,黄珠琪,古锦超,李光亚,刘爱军,潘浩,曹佳会,秦秀德..石菖蒲挥发油活性成分甲基丁香酚靶向JAK2/STAT3通路缓解脑微血管内皮细胞炎症和焦亡[J].南京中医药大学学报,2026,42(6):870-881,12.基金项目
国家自然科学基金面上项目(32000551) （32000551）
深圳市科技计划项目(JCYJ20230807094817035) （JCYJ20230807094817035）
广州市科技计划项目青年博士"启航"项目(2025A04J4268) （2025A04J4268）
广州中医药大学校院联合科技创新基金(GZYFT202402) （GZYFT202402）
