农业生物技术学报2026，Vol.34Issue(7)：1495-1505,11.DOI:10.3969/j.issn.1674-7968.2026.07.012
叔丁基对苯二酚通过抗氧化应激和抗凋亡作用减轻T-2毒素诱导的雄性大鼠肾毒性
Tertiary Butylhydroquinone(TBHQ)Mitigates T-2 Toxin-induced Nephrotoxicity in Male Rats(Rattus norvegicus)via Anti-oxidative Stress and Anti-apoptotic Effects
摘要
Abstract
T-2 toxin is a highly toxic Fusarium toxin commonly found in grains and feed.It can activate apoptosis pathways by inducing oxidative stress and mitochondrial dysfunction,and damage important organs.The kidney,as a key organ for toxin metabolism and excretion,is more vulnerable to attack.However,effective protective strategies against its nephrotoxicity are still limited.As a phenolic antioxidant,tertiary butylhydroquinone(TBHQ)can activate the Nrf2/HO-1 pathway and counteract oxidative stress and apoptosis.Therefore,this study aims to investigate the protective effect of TBHQ against T-2 toxin-induced nephrotoxicity in male rats and to explore its molecular mechanisms.40 male SD rats(Rattus norvegicus)were divided into Control group,T-2 group(T-2 toxin only),TBHQ+T-2 group(TBHQ i.p.for 14 d,followed by T-2 gavage for 7 d),and M group(simultaneous TBHQ and T-2 for 7 d).End-point analysis included serum markers(SCR,Cys-C,β2-MG),renal oxidative stress(SOD,CAT,GSH-PX,TAC,MDA),antioxidant gene expression(Sod,Cat,Gpx),histopathology(HE,Masson,PAS staining),and the cell apoptosis rate(TUNEL).The results showed that compared with Control group,serum levels of SCR and Cys-C were significantly elevated in rats of the T-2 group(P＜0.05);renal SOD and MDA contents as well as the renal cell apoptosis rate were significantly increased(P＜0.05),accompanied by damage to renal structure.However,TBHQ pretreatment significantly reversed these pathological changes:It reduced serum renal markers and MDA content(P＜0.05),markedly reduced cell apoptosis(P＜0.01),significantly improved histopathological lesions,and upregulated the mRNA expression of antioxidant genes Gpx(P＜0.05)compared with the T-2 group.Furthermore,the protective effect of the TBHQ pretreatment regimen was superior to the M group.In conclusion,TBHQ mitigated T-2 toxin-induced renal injury in male rats by enhancing its anti-oxidative stress and anti-apoptotic effects,with pretreatment offering a more effective strategy.This study provides experimental evidence for elucidating the oxidative stress apoptosis mechanism of T-2 toxin nephrotoxicity as well as theoretical references for preventive protective measures against feed mycotoxin related kidney injury.关键词
叔丁基对苯二酚(TBHQ)/T-2毒素/肾脏损伤/雄性大鼠/凋亡Key words
Tertiary butylhydroquinone(TBHQ)/T-2 toxin/Kidney injury/Male rat/Apoptosis分类
农业科技引用本文复制引用
陈淑妍,黎源,刘雨晴,陈丽圆,陈清桦,唐胜球,陈云..叔丁基对苯二酚通过抗氧化应激和抗凋亡作用减轻T-2毒素诱导的雄性大鼠肾毒性[J].农业生物技术学报,2026,34(7):1495-1505,11.基金项目
省级大学生创新创业训练计划(S202410576034) （S202410576034）
广东省基础与应用基础研究基金(2024A1515013150) （2024A1515013150）
广东省普通高校特色创新项目(2024KTSCX068) （2024KTSCX068）
2023年度韶关市支持科研工作者项目(230324108035353) （230324108035353）
2024年度韶关市支持科研工作者项目(P0000876050) （P0000876050）
