热带作物学报2026，Vol.47Issue(6)：1543-1552,10.DOI:10.3969/j.issn.1000-2561.2026.06.016
MAPK激酶EqFus3在橡胶树白粉菌寄主识别和侵染过程中的功能研究
Functional Study of MAPK Kinase EqFus3 in Host Recognition and Infection Process of Rubber Tree Powdery Mildew Fungus Erysiphe quercicola
摘要
Abstract
The MAPK kinase Fus3 plays an important regulatory role in host signal recognition and pathogenic proc-esses of various pathogenic fungi,yet its functions in powdery mildew fungi remains uncharacterized.Erysiphe querci-cola,the causal agent of rubber tree powdery mildew,is a pathogenic fungus infecting rubber trees(Hevea brasiliensis),and this study focused on the functions of EqFus3 in host signal recognition and pathogenicity of this pathogen.Gene expression analysis demonstrated that EqFus3 was significantly up-regulated at the early infection stage and reached its expression peak at 13 hours post-inoculation.The dsRNA-mediated silencing of EqFus3 in fungal spores at the time of inoculation resulted in a marked decrease in fungal invasion rate and attenuated pathogenicity.Exogenous application of cellulose and potassium ions enabled the pathogen to form hyphae and haustoria on glass slides(a non-plant surface),which suggested that cellulose(a major component of plant cell walls)and environmental potassium ions may act as signal molecules for the pathogen to recognize and induce the formation of infection structures.Notably,dsRNA-mediated silencing of EqFus3 abrogated the inductive effects of cellulose and potassium ions on the pathogen,indicating that EqFus3 is involved in the recognition of host-derived and environmental signals.This study clarifies the functional role of Fus3 kinase in host recognition and infection processes of the pathogen.关键词
橡胶树/白粉菌/MAPK激酶/EqFus3Key words
rubber tree/Erysiphe quercicola/MAPK kinase/EqFus3分类
农业科技引用本文复制引用
吕焱洋,殷金瑶,朱雪嬛,陈亚龙,缪卫国,李潇..MAPK激酶EqFus3在橡胶树白粉菌寄主识别和侵染过程中的功能研究[J].热带作物学报,2026,47(6):1543-1552,10.基金项目
海南省自然科学基金项目(No.324MS015) （No.324MS015）
海南省科技人才创新项目(No.KJRC2023B14). （No.KJRC2023B14）
