宁夏农林科技2026，Vol.67Issue(5)：1-8,67,9.DOI:10.3969/j.issn.1002-204x.2026.05.001
宁夏枸杞内生菌分离和鉴定
Isolation and Identification of Endophytic Microorganisms in Lycium barbarum L.
摘要
Abstract
Lycium barbarum L.is an important medicinal and edible plant in China,rich in endophytic microbial resources.Taking the tissue culture seedlings of'Ningqi No.1'as the research object,through methods such as endophytic microbial isolation,cultivation purification,colony morphological observation,DNA extraction,PCR amplification,sequencing and comparison with the NCBI database,the endophytic microbial community of these seedlings was systematically isolated and identified.The differences in the endophytic microbial communities among different growth stages of the plants were also compared.Through isolation and identification,a total of 7 endophytic microorganisms were obtained.The composition characteristics of the microbial community showed that Alternaria Nees(relative abundance 38.2%)was the dominant fungus,and Sphingomonas(relative abundance 19.1%)was the dominant bacterium.It was also found that the abundance of Sphingomonas and Methylobacterium was closely related to the growth vigor of the host,and the detection rate of these microorganisms in the"dominant individual"(11.7%)was significantly higher than that in the"inferior individual"(4.3%).The research results indicated that the tissue culture seedlings of'Ningqi No.1'contained abundant endophytic microbial resources,and their community structure,especially the abundance of beneficial microorganisms,was closely related to the growth vitality of the host.This lays a theoretical foundation for in-depth research on the symbiotic relationship between endophytic microorganisms and Lycium barbarum and the development of new microbial agents.关键词
宁夏枸杞/内生菌/甲基杆菌Key words
Lycium barbarum L./Endophytic microorganisms/Methylobacterium分类
农业科技引用本文复制引用
高燕,樊云芳,覃理彬,张豪,王丹青,秦垦..宁夏枸杞内生菌分离和鉴定[J].宁夏农林科技,2026,67(5):1-8,67,9.基金项目
宁夏回族自治区农业高质量发展和生态保护科技创新示范课题"宁杞1号提纯复壮与退化机制研究"(NGSB-2021-2-02)、宁夏回族自治区重点研发计划项目"宁夏枸杞病毒病害入侵风险评估与绿色防控关键技术研究"(2025BBF02003)、银川市科技支撑专项"枸杞脱毒种苗繁育技术体系的构建及应用"(2025NY09)、宁夏回族自治区财政林业新技术引进及推广项目"枸杞优新品系筛选及配套栽培技术示范"(2026ZY01). （NGSB-2021-2-02）
