菌物学报2026，Vol.45Issue(6)：165-177,13.DOI:10.13346/j.mycosystema.250304
北京地区羊肚菌白霉病病原及其生物学特性
Pathogen of white mold disease of Morchella sextelata in Beijing and its biological characteritics
摘要
Abstract
The pathogen of fungal diseases endangering Morchella sextelata in Beijing was isolated and purified by using tissue isolation method.Morphological and molecular biology methods were used for identification of the pathogen.Suitable growing temperatures and pH,and the effects of carbon and nitrogen sources on the growth rate,colony morphology,and sporulation of fungal hyphae were systematically analyzed.Through morphological observation and analysis of ITS,RPB2,and LSU gene sequences,the pathogen was identified as Pseudodiploospora longispora(similarity>99%).The Koch's rule inoculation test verified the pathogenicity of the pathogen.It was confirmed that the optimal growth temperature of the fungus was 20℃,and the pH adaptation scope was wide,ranging from pH 5 to pH 9.The most suitable carbon source is sucrose,and the most suitable nitrogen source is malt extract powder.Under the conditions of 20℃and pH 5,the highest sporulation was attained by using glucose as the carbon source and yeast extract as the nitrogen source.This test provides theoretical reference for preventing and controlling the white mold disease of cultivated Morchella sextelata.关键词
羊肚菌/真菌病害/分离鉴定/长孢假单隔孢/生物学特性Key words
Morchella/fungal disease/isolation and identification/Pseudodipolospora longispora/biological characteristics引用本文复制引用
曲珈萱,张亚静,张殿朋,王守现,刘宇,律凤霞,卢彩鸽..北京地区羊肚菌白霉病病原及其生物学特性[J].菌物学报,2026,45(6):165-177,13.基金项目
现代农业产业技术体系北京市食用菌创新团队(BAIC03) （BAIC03）
国家食用菌产业技术体系(CARS20) This work was supported by the Beijing Edible Fungi Innovation Team of the Modern Agricultural Industrial Technology System(BAIC03)and the National Edible Fungi Industry Technology System(CARS20). （CARS20）
