菌物学报2026，Vol.45Issue(6)：229-241,13.DOI:10.13346/j.mycosystema.250293
玫红脉柄牛肝菌液体菌种培养基优化及胞外酶活性变化特征
Optimization of liquid spawn medium and variation characteristics of extracellular enzyme activities of Phlebopus roseus
摘要
Abstract
The wild strain 2019MYKLR003 of Phlebopus roseus was used as the test material.Basic parameters for submerged culture were determined through single-factor experiments,and the medium formula was optimized using the response surface methodology.A high-activity induction formula was designed and verified,and the activity changes of peroxidase,laccase,and cellulase in mycelia under different fermentation conditions were determined.The results showed that this strain had a high oxygen demand for submerged culture and was highly sensitive to pH changes.The optimal pH value was 5 and liquid volume was 100 mL.The optimal medium formula(RA1)consisted of 432.35 g/L potato extract,2.00 g/L tryptone,and 32.47 g/L mannitol.The actual measured mycelial biomass increased by 130.21%as compared with the predicted value.The addition of 100 μg/mL VB1,50 μg/mL VB2,50 μg/mL VB3,and 5 g/L carboxymethyl cellulose to RA1 significantly alleviated mycelial browning and led to regular and uniform mycelial morphology.Adjusting the fermentation endpoint to 18 days could achieve the maximum mycelial biomass synthesis while ensuring the simultaneous highest activities of the three extracellular enzymes.This study provides a research reference for the standardized production of high-activity submerged spawn of P.roseus.关键词
玫红脉柄牛肝菌/响应面法/发酵培养/胞外酶Key words
Phlebopus roseus/response surface methodology/submerged fermentation culture/extracellular enzyme引用本文复制引用
方亮,柳成益,孔令加,高院妮,黄中华,刘斌,杨梅..玫红脉柄牛肝菌液体菌种培养基优化及胞外酶活性变化特征[J].菌物学报,2026,45(6):229-241,13.基金项目
四川省重点研发计划项目(2023YFN0039) （2023YFN0039）
四川省食用菌创新团队项目(SCCXTD-2025-07) This work was supported by the Key Research and Development Program Project of Sichuan Province(2023YFN0039)and the Sichuan Edible Fungi Innovation Team Project(SCCXTD-2025-07). （SCCXTD-2025-07）
