疏水蛋白基因Cmhyd4对蛹虫草生长发育及基因表达的调控
Regulation of hydrophobin gene Cmhyd4 on the growth,development and gene expression of Cordyceps militaris
摘要
Abstract
Hydrophobins,small secreted proteins unique to filamentous fungi,play critical roles in fungal growth and development yet their functional mechanisms are poorly understood.Previously,a hydrophobin gene Cmhyd4 knockout strain ∆Cmhyd4cas9 of Cordyceps militaris was generated via gene editing.A comparative analysis of mycelial growth and fruiting body development before and after Cmhyd4 deletion was conducted.It was revealed that the loss of Cmhyd4 promoted aerial hyphae growth,and increased the number of fruiting bodies and biological conversion efficiency per cultivation bottle.Using wild-type(WT)and ∆Cmhyd4cas9 strains as experiment materials,samples from the mycelia(MY),primordium(PR),and mature fruiting body(MF)were subjected to comparative transcriptomic analysis.The results showed that Cmhyd4 deletion altered gene transcript levels across all stages as compared with WT strain.The highest differentially expressed genes(DEGs)occurred at PR stage(349),and MY stage(179)was the next,while the fewest at MF stage(93).The transcriptional patterns of hydrophobin family genes at different developmental stages provided critical insights into their functions.The deletion of Cmhyd4 altered the transcription levels of the other family members,demonstrating significant gene compensation effects.GO enrichment analysis revealed that after Cmhyd4 gene deletion,DEGs at the MY stage were primarily associated with cell cycle and mitosis,whereas DEGs at PR and MF stages were linked to abiotic stress response.Consisted with GO analysis,the ∆Cmhyd4cas9 strain exhibited significant enhancement of NaCl stress resistance in fruiting bodies,while no notable changes were observed in the mycelial stage.RT-qPCR validation confirmed that four genes in the osmotic stress response pathway showed no differential expression at MY stage after Cmhyd4 gene deletion,but significant upregulation occurred in PR and MF stages.This study comprehensively elucidates the impacts of Cmhyd4 on the growth and development of C.militaris,along with its regulatory role in gene expression,providing genetic resources for future molecular breeding of C.militaris.关键词
蛹虫草/菌丝/子实体/非生物胁迫/基因Key words
Cordyceps militaris/mycelium/fruiting body/abiotic stress/gene引用本文复制引用
任慧慧,王佳媛,李忞阳,刘苏雅,张婕,马敖,李守勉,李肖..疏水蛋白基因Cmhyd4对蛹虫草生长发育及基因表达的调控[J].菌物学报,2026,45(6):276-289,14.基金项目
国家自然科学基金(32202569) （32202569）
河北省自然科学基金(C2023204076) （C2023204076）
河北农业大学创新创业训练计划项目(2026070) （2026070）
河北省科技计划项目重点研发计划(21326315D) （21326315D）
河北省省属高等学校基本科研业务费研究项目(KY2023055) （KY2023055）
河北农业大学引进人才科研专项(YJ202249) This work was supported by the National Natural Science Foundation of China(32202569),the Natural Science Foundation of Hebei Province(C2023204076),the Innovation and Entrepreneurship Training Program Project of Hebei Agricultural University(2026070),the Key Research and Development Planning Project in Science and Technology of Hebei Province(21326315D),the Research Project of Basic Scientific Research Expenses of Colleges and Universities in Hebei Province(KY2023055),and the Talent Introduction Scientific Research Special Project of Hebei Agricultural University(YJ202249). （YJ202249）
