特产研究2026，Vol.48Issue(3)：57-61,67,6.DOI:10.16720/j.cnki.tcyj.2025.079
貂源肺炎克雷伯菌毒力基因及耐药特征分析
Analysis of Virulence Genes and Drug Resistance Characteristics of Klebsiella pneumoniae Isolated from Mink
摘要
Abstract
Klebsiella pneumoniae can cause pneumonia in mink.It has seriously affected the development of mink breeding industry.The purpose of this study was to analyze the virulence and drug resistance of Klebsiella pneumoniae from mink,and to provide thearetical basis for investigating the pathogenicity and drug resistance of Klebsiella pneumoniae.In this study,the bacteria were isolated and identified by pure culture,gram staining and PCR method.The agar microdilution method was used to detected the drug resistance spectrum of the strain.The serotype was identified by PCR method.The whole genome DNA was extracted and sequenced.The composition and important com-ponents of the whole genome sequence were analyzed.The genotypes,drug resistance genes and virulence genes of the strain were analyzed by bioinformatics of whole genome.The results showed that the isolated strain was Klebsiella pneumoniae,which belonged to K2 serotype.The results of drug sensitivity test suggested that the strain was resistant to tetracycline,chloramphenicol,florfenicol,ampicillin,ce-ftazidime,cefoxitin,ciprofloxacin,enrofloxacin and kanamycin,showing multiple drug resistance.The assembled genome size was 5435.522 kb and contained one chromosome and one plasmid.The whole genome analysis showed that the strain belonged to ST86.The strain carried 7 virulence genes and 6 resistance genes.In this study,one Klebsiella pneumoniae strain was isolated.Serotype,drug sensi-tivity test and whole genome analysis showed that the strain was highly virulent and multi-drug resistant,which was related to the high pa-thogenicity of Klebsiella pneumoniae and was easy to cause infection in humans and animals.关键词
水貂/肺炎克雷伯菌/毒力基因/耐药基因/全基因组Key words
mink/Klebsiella pneumoniae/virulence gene/drug resistance gene/whole genome分类
农业科技引用本文复制引用
陈强,程悦宁,王咸伟,刘菘岩,付连军,朱站国,孙娜..貂源肺炎克雷伯菌毒力基因及耐药特征分析[J].特产研究,2026,48(3):57-61,67,6.基金项目
吉林省教育厅科学技术研究项目(JJKH20220401KJ) （JJKH20220401KJ）
