水生生物学报2026，Vol.50Issue(7)：10-17,8.DOI:10.3724/1000-3207.2025.2025.0429
脯氨酸羟化酶phd1的缺失增强斑马鱼的低氧耐受能力
DELETION OF PROLYL HYDROXYLASE 1(PHD1)IN ZEBRAFISH FACILITATES HYPOXIA TOLERANCE
摘要
Abstract
This study aims to elucidate the role of proline hydroxylase phd1 in hypoxia tolerance in zebrafish.To this end,wild-type(phd1+/+)and phd1-deficient(phd1-/-)zebrafish were subjected to hypoxic conditions.Then,erythrocyte numbers were analyzed using o-dianisidine staining,and hypoxia-responsive gene expression was assessed via real-time quantitative PCR.Additionally,the survival of zebrafish larvae and adults was measured.The study initially vali-dated the conservation of the PHD1 protein in humans,mice,and zebrafish.Under hypoxic conditions,the number of erythrocytes in phd1-/-zebrafish increased significantly compared to phd1+/+zebrafish,and phd1-/-zebrafish larvae and adults exhibited enhanced tolerance to hypoxia.Furthermore,hypoxia-responsive genes were more strongly induced in phd1-/-zebrafish than in phd1+/+zebrafish.These findings suggest that knocking out phd1 enhances hypoxia tolerance in zebrafish,advancing our understanding of the biological function of this gene in fish and offering a molecular target for breeding hypoxia-tolerant fish strains.关键词
脯氨酸羟化酶/phd1/基因敲除/低氧耐受/斑马鱼Key words
Prolyl hydroxylase/phd1/Gene knock-out/Hypoxia tolerance/Zebrafish分类
生物科学引用本文复制引用
化家乐,邓洪艳,王子旋,罗怡曼,肖武汉,程祎斌,刘兴..脯氨酸羟化酶phd1的缺失增强斑马鱼的低氧耐受能力[J].水生生物学报,2026,50(7):10-17,8.基金项目
湖北省自然科学基金(2022CFA110) （2022CFA110）
国家重点研发计划(2022YFF1000302) （2022YFF1000302）
国家资助博士后研究人员计划(GZB20250721)资助[Supported by the Natural Science Foundation of Hubei Province of China(2022CFA110) （GZB20250721）
the National Key Research and Development Program of China(2022YFF1000302) （2022YFF1000302）
the Postdoctoral Fellowship Program(Grade B)of China Postdoctoral Science Foundation(GZB20250721)] （Grade B）
