水生生物学报2026，Vol.50Issue(7)：51-61,11.DOI:10.3724/1000-3207.2026.2026.0019
温度对克氏原螯虾摄食、生长、酶活性及肠道菌群的影响
TEMPERATURE ON FEEDING,GROWTH,ENZYME ACTIVITY,AND INTESTINAL MICROBIOTA OF PROCAMBARUS CLARKII
摘要
Abstract
To investigate the effects of temperature on the feeding,growth,enzyme activity,and intestinal microbiota of the red swamp crayfish(Procambarus clarkii),juveniles with an average weight of(3.80±0.05) g were selected and subjected to four temperature treatments(15℃,20℃,25℃,and 30℃).The results indicated that both daily feed intake and daily feeding rate increased with rising temperature,with the 30℃group exhibiting a significantly higher feeding rate compared to the 15℃and 20℃groups.In terms of growth,the 25℃group achieved the highest weight gain and specific growth rate,significantly surpassing the 15℃group,while the 30℃group displayed the highest molting rate and was also significantly higher than the 15℃group.Enzyme activity analysis revealed that the 25℃group showed significantly higher activities of digestive enzymes(protease and amylase)than the other temperature groups.Additio-nally,this group demonstrated significantly elevated levels of antioxidant(total antioxidant capacity,superoxide dismu-tase,catalase)and nonspecific immune enzymes(acid phosphatase,alkaline phosphatase)compared to the other groups,indicating enhanced stress resistance and immune capacity at 25℃.Intestinal microbiota analysis indicated that the 20℃group had the highest microbial community richness(Observed OTUs,Chao1,Ace indices).However,at 25℃,significant alterations in community structure were observed at the phylum and genus levels:the relative abundances of Patescibacteria,Planctomycetota,and Bdellovibrionota decreased significantly.At the genus level,the beneficial Lysinibacillus increased significantly,while potential pathogens such as Candidatus Bacilloplasma,Shewanella,Pseu-domonas,and Pseudoxanthomonas decreased significantly.These findings suggest that 25℃is conducive to the growth of P.clarkii,enhancing its digestive enzyme activity,immune function,disease resistance,and significantly improving the intestinal microbiota structure.关键词
温度/摄食/生长/酶活性/肠道菌群/克氏原螯虾Key words
Temperature/Feeding/Growth/Enzyme activity/Intestinal microbiota/Procambarus clarkii分类
农业科技引用本文复制引用
郭铠莹,王宇驰,徐登歌,荣宽,郭蕊,郭超,刘家寿,张堂林,李为..温度对克氏原螯虾摄食、生长、酶活性及肠道菌群的影响[J].水生生物学报,2026,50(7):51-61,11.基金项目
国家重点研发计划(2023YFD2400505和2023YFD2401802) （2023YFD2400505和2023YFD2401802）
吉安市创新类人才项目(jasb202310324) （jasb202310324）
黄河三角洲产业领军人才工程(DYRC20200215)资助[Supported by the National Key R&D Program of China(2023YFD2400505 and 2023YFD2401802) （DYRC20200215）
the Innovative Talents Project of Ji'an city(jasb202310324) （jasb202310324）
the Yellow River Delta Industry Leading Talent Project(DYRC20200215)] （DYRC20200215）
