湖北科技学院学报（医学版）2026，Vol.40Issue(3)：185-189,5.DOI:10.16751/j.cnki.2095-4646.2025120606
百合水提物对皮质酮诱导的SH-SY5Y细胞损伤模型的保护作用及其机制
Protective Effects of Aqueous Extract of Lilii Bulbus on a Corticosterone-Induced SH-SY5 Y Cell Injury Model and Its Underlying Mechanisms
许梦莹 1寇真真2
作者信息
- 1. 郑州市第九人民医院药学部,河南 郑州 450003
- 2. 绍兴市上虞区中医医院中药药剂科
- 折叠
摘要
Abstract
Objective To investigate the effects of an aqueous extract of Lilii Bulbus(AELB)on the growth of SH-SY5 Y cells,and its neuroprotective role against corticosterone(CORT)-induced neuronal injury in these cells.Methods SH-SY5 Y cells were pretreated with various concentrations of AELB for 8h,followed by exposure to 10μmol/L CORT for 24h to establish an in vitro neuronal injury model.Cell viability was assessed using the MTT assay,and the protein levels of brain-derived neurotrophic factor(BDNF)and nerve growth factor(NGF)were measured by Western blotting.Results AELB(6.25～800μg/mL)exhibited no cytotoxicity toward normal SH-SY5 Y cells.CORT(10μmol/L)significantly reduced cell viability,whereas pretreatment with AELB(100 and 200μg/mL)for 8h markedly improved cell viability.Both BDNF and NGF protein levels were significantly downregulated in CORT-injured cells but were dose-dependently restored by AELB(50,100,and 200μg/mL).Conclusion AELB demonstrates notable neuroprotective effects against CORT-induced SH-SY5 Y cell injury,and its underlying mechanism may be closely associated with its regulation of neurotrophic factor signaling pathways.关键词
百合水提物/SH-SY5Y细胞/神经保护/神经营养因子Key words
Aqueous extract of Lilii Bulbus/SH-SY5 Y cells/Neuroprotection/Neurotrophic factors分类
医药卫生引用本文复制引用
许梦莹,寇真真..百合水提物对皮质酮诱导的SH-SY5Y细胞损伤模型的保护作用及其机制[J].湖北科技学院学报（医学版）,2026,40(3):185-189,5.
