畜牧与兽医2026，Vol.58Issue(7)：88-98,11.
一株类NADC30/HP-PRRSV重组毒株HNxy23的分离鉴定与遗传演化分析
Isolation,identification,and genetic and evolutionary analysis of a recombinant NADC30-like/HP-PRRSV strain HNxy23
摘要
Abstract
To investigate the epidemiological status and genetic variation of porcine reproductive and respiratory syndrome virus(PRRSV)in Henan Province,lung tissue samples obtained from a suspected PRRSV-positive pig farm in Xinyang were tested and subjected to virus isolation.PRRSV was successfully isolated on MARC-145 cells and designated as HNxy23.Subsequent sequence analysis of HNxy23 re-vealed the following facts:The full length of the HNxy23 genome was 15 049 bp.It possessed a homology of 84.1%with the classic American strain VR-2332,and had homologies of 83.6%,90.2%,90.2%,and 91.0%with JXA1,NADC30,CHsk1401,and 15LN1,respectively.The amino acid sequence alignment of Nsp2 showed that HNxy23,similar to the NADC30 strain,had a total of 131 discontinuous amino acid site deletions,including 111+1+19.GP5 amino acid sequence alignment revealed partial amino acid mutations in the antigenic epitopes of HNxy23.The recombination analysis confirmed HNxy23 to be a recombinant strain between lineage 1 NADC30 and lineage 8 highly pathogen-ic vaccine strain JXA1-P80,with a recombination region of 5 392 bp to 6 969 bp.关键词
猪繁殖与呼吸系统综合征病毒(PRRSV)/河南省/类NADC30/毒株分离/重组分析Key words
porcine reproductive and respiratory syndrome virus(PRRSV)/Henan Province/NADC30-like/virus isolation/recombina-tion analysis分类
农业科技引用本文复制引用
刘恒岩,毛思洋,王思宇,廖廷堯,王丽,顾伟伟,杨俊静..一株类NADC30/HP-PRRSV重组毒株HNxy23的分离鉴定与遗传演化分析[J].畜牧与兽医,2026,58(7):88-98,11.基金项目
大学生创新训练计划项目(SIPT项目) （SIPT项目）
