西南林业大学学报2026，Vol.46Issue(5)：133-141,9.DOI:10.11929/j.swfu.202503053
云南'兔眼'蓝莓叶内生真菌多样性分析
Diversity Analysis of Endophytic Fungi in'Rabbit-Eye'Blueberry Leaves from Yunnan
摘要
Abstract
This study investigated the community composition and diversity of endophytic fungi in the leaves of"Rabbit-eye"blueberries from Lanping,Dali,and Jingdong in Yunnan Province through Illumina high-through-put sequencing.It also explored the effects of ecological and climatic factors,as well as cultivation management practices,on the community structure of these fungi and predicted their potential ecological functions based on the FUNGuild database.Results showed that a total of 663 OTUs were detected across all samples,classified into 8 phyla,31 classes,89 orders,187 families,289 genera,and 389 species.Thirtee OTUs were shared among all re-gions,with the number of unique OTUs following the order:Dali＞Lanping＞Jingdong.The alpha diversity and richness of the endophytic fungal communities were the highest in Dali and lowest in Jingdong.At the phylum level,Ascomycota was dominated in Dali(82.13％)and Lanping(88.17％),while the dominant phylum in Jing-dong was unclassified fungi(Fungi_phy_Incertae_sedis)(40.92％).At the genus level,Cladosporium(38.99％)and Leptosphaerulina(13.02％)were the dominant genera in Lanping,Epicoccum(20.81％)and Cladosporium(9.66％)in Dali,and unclassified fungi and unknown fungi(unclassified_k__Fungi)accounted for 60.82％of the community in Jingdong.Significant differences in the relative abundance of the top 30 genera were observed among the regions.Functional prediction using the FUNGuild database revealed that the Pathotroph-Saprotroph-Symbiotroph functional group was predominant in Lanping(44.65％),the unknown nutritional group in Jingdong(61.6％),and the Pathotroph-Saprotroph group in Dali(34.74％).The annual average temperature and plastic film-covered cultivation had opposite effects on the relative abundance of the dominant genera compared with other environmental factors.These 2 factors were positively correlated with the relative abundance of 5 genera,includ-ing Trichosporon,and negatively correlated with 6 genera,including Cladosporium.In contrast,9 genera,includ-ing Rhodotorula,were not significantly affected by ecological and climatic factors or cultivation practices,sug-gesting broader survival adaptability.In conclusion,significant differences in the community composition,di-versity,and ecological functions of endophytic fungi in"Rabbit-eye"blueberry leaves were observed across dif-ferent regions in Yunnan.Ecological and climatic factors,as well as cultivation management practices had dis-tinct impacts on the community structure of these fungi.关键词
'兔眼'蓝莓/高通量测序/内生真菌/微生物多样性Key words
'Rabbit-eye'blueberry/high-throughput sequencing/endophytic fungi/microbial diversity分类
农业科技引用本文复制引用
苏发辉,张丽兴,王状,罗宗龙,冯源..云南'兔眼'蓝莓叶内生真菌多样性分析[J].西南林业大学学报,2026,46(5):133-141,9.基金项目
云南省科技厅科技计划项目-地方高校联合专项项目(202001BA070001-210)资助. （202001BA070001-210）
