针刺研究2026，Vol.51Issue(6)：781-788,8.DOI:10.13702/j.1000-0607.20250407
筛选穴位局部刺激关联神经环路的现代研究技术进展
Advances of application of modern techniques for screening the neural circuits associated with local stimulation of acupoints
摘要
Abstract
It is well known that acupoints have an intricate connection with the nervous system.The specific mechanism of acupuncture in regulating the nervous system and exerting the therapeutic effect is one of the hottest and most difficult aspects in the field of acupuncture research.The regulation of acupuncture on the nervous system involves multi-target and multi-link neurofeedback,and how to find the critical effector neurons in this process is the primary concern.In the present article,we introduce some modern paradigms and methodologies for screening acupuncture-responsive effector neurons starting from elucidation of structural and functional connectivity between acupoints and neurons,for example,1)neurovirus tracing techniques for studying the neuronal structural connections,and immediate early gene labeling for identifying functionally connected neuronal ensembles activated by acupuncture stimulation,2)neuroimaging techniques for observing the impact of acupuncture on brain functional connections from a macroscopic perspective in real-time,3)in vitro and in vivo electrophysiological recordings,as well as in vivo fiber-optics recording or two-photon calcium imaging for revealing the activity characteristics of activated neuronal ensembles,4)analysis of the causal relationship between the key effect neurons,their neural circuit connections and the therapeutic effect of acupuncture by using multiple measures such as optogenetics and chemogenetics,etc.We should further strengthen the connection between the experimental research,basic theory exploration and clinical practice of acupuncture,accelerate the process of revealing the scientific connotations and promote the modernization and internationalization of acupuncture therapy.关键词
针刺/穴位/神经环路/神经示踪/现代技术Key words
Acupuncture/Acupoint/Neural circuit/Neural tracing/Modern techniques引用本文复制引用
狄文惠,黄可妮,易立琛,龙浩曦,许能贵,姚露露..筛选穴位局部刺激关联神经环路的现代研究技术进展[J].针刺研究,2026,51(6):781-788,8.基金项目
国家自然科学基金面上项目(No.82474623、82374573) （No.82474623、82374573）
国家自然科学基金重点项目(No.82430125) （No.82430125）
