中国农学通报2026，Vol.42Issue(9)：138-144,7.
甜茶组培快繁技术体系的建立
Establishment of Rubus chingii var.suavissimus Tissue Culture Rapid Propagation Technology System
摘要
Abstract
The paper aims to develop a rapid propagation system for the medicinal resource Rubus chingii var.suavissimus.Using tender stem segments with buds as explants,the study investigated the effects of sterilization duration,hormone concentration ratios in subculture proliferation media and rooting media,as well as transplanting substrate ratios on aseptic line establishment,cluster bud proliferation,test-tube plantlet rooting,and transplant growth of tissue-cultured seedlings.Orthogonal test design combined with stepwise regression analysis was used to screen the optimal conditions of each link.Results showed that the optimal sterilization method for explants was treatment with 0.1%HgCl2 solution for 7 minutes,achieving a survival rate of 80%.The optimal subculture proliferation medium was MS+6-BA 1.0 mg/L+NAA 0.1 mg/L,with a proliferation coefficient of 27.33.The optimal rooting medium was 1/2MS+IBA 0.5 mg/L,resulting in 100%rooting rate,approximately 13 average roots per plant,and average root length of 1.74 cm.The optimal transplanting substrate ratio was vermiculite:nutrient soil:coconut coir=1:1:1,with a survival rate as high as 86.3%.The successful establishment of a rapid tissue culture propagation system for Rubus chingii var.suavissimus holds significant importance for the sustainable development of sweet tea resources and the conservation of genetic diversity in characteristic medicinal plants.关键词
甜茶/覆盆子/组织培养/快速繁殖/离体再生/生长调节剂/增殖/生根Key words
Rubus chingii var.suavissimus/Rubus chingii/tissue culture/rapid propagation/in vitro regeneration/plant growth regulator/proliferation/rooting分类
农业科技引用本文复制引用
张亚斐,张成才,代晓雨,张小波,王升,乔萍,汪奕衡,孙嘉惠..甜茶组培快繁技术体系的建立[J].中国农学通报,2026,42(9):138-144,7.基金项目
科技基础资源调查专项"具有潜在药用价值的中药资源调查"(2022FY101004) （2022FY101004）
中国中医科学院科技创新工程项目资助(CI2026A039) （CI2026A039）
中国中医科学院科技创新工程项目"道地药材五味子、覆盆子、黄连的药用植物多样性与进化"(CI2023E002) （CI2023E002）
国家中医药管理局高水平中医药重点学科建设项目"中药资源生态学"(zyyzdxk-2023244) （zyyzdxk-2023244）
财政部和农业农村部国家现代农业产业技术体系(CARS-21). （CARS-21）
