中国农学通报2026，Vol.42Issue(9)：145-154,10.
花生胚小叶高效离体再生体系的建立与优化
Arachis hypogaea L.Embryo Leaflet:Establishment and Optimization of in Vitro Regeneration System
摘要
Abstract
The purpose of this study was to establish an efficient in vitro regeneration system of embryo lobules of different peanut varieties,and to provide strong technical support for genetic improvement and biotechnology application of peanut.In this experiment,the embryo lobules of two different peanut varieties'Laiyang Silihong'and'83'('Yunhua 24')were used as explants,and the effects of different plant growth regulators and seedling hardening time on the establishment of embryo lobule regeneration system were studied by tissue culture method.The results showed that the most suitable growth regulator for'Laiyang Silihong'was 3.25 mg/L 6-BA+0.2 mg/L NAA for bud cluster induction,1.5 mg/L TDZ+3.0 mg/L 6-BA+1.0 mg/L GA3 for bud cluster extraction,2.0 mg/L TDZ+4.0 mg/L 6-BA+0.1 mg/L NAA+1.5 mg/L GA3 for bud cluster elongation,and 2.0 mg/L NAA+0.3 mg/L 2,4-D for rooting culture.The most suitable growth regulator for'83'('Yunhua 24')embryo leaflet bud induction was 3.25 mg/L 6-BA+0.3 mg/L NAA,the bud cluster was 1.5 mg/L TDZ+4.0 mg/L 6-BA+1.5 mg/L GA3,the bud cluster elongation was 2.0 mg/L TDZ+3.5 mg/L 6-BA+1.0 mg/L NAA+2.0 mg/L GA3,and the rooting culture was 2.5 mg/L NAA+0.1 mg/L 2,4-D.The best transplanting time of the two peanut varieties was 7 days,and the fruit setting rates of'Laiyang Silihong'and'83'('Yunhua 24')were 57.63%and 48.67%,respectively.In this study,the in vitro regeneration system of embryo leaflets of two peanut varieties,'Laiyang Silihong'and'83'('Yunhua 24'),was successfully established.The results not only improved the peanut tissue culture technology system,but also provided important transgenic technology platform support for subsequent peanut genetic improvement research and functional gene mining.关键词
花生/胚小叶芽丛/离体再生体系/组织培养法/植物生长调节剂Key words
peanut/embryo leaflet buds/in vitro regeneration system/tissue culture method/plant growth regulators分类
农业科技引用本文复制引用
李婷婷,张建成,迟晓元,姜骁,刘晓芹..花生胚小叶高效离体再生体系的建立与优化[J].中国农学通报,2026,42(9):145-154,10.基金项目
山东省重点研发计划"基于大数据与基因编辑的野生花生高产抗病性状驯化及种质创制"(2024LZGC035) （2024LZGC035）
泰山学者工程资助"花生分子设计育种与种质资源创新"(tsqn2021031610) （tsqn2021031610）
潍坊市科技发展计划项目"花生重要农艺性状调控基因挖掘与育种应用"(2024JZ0016) （2024JZ0016）
财政部和农业农村部:国家现代农业产业技术体系"抗逆品种改良"(CARS-13). （CARS-13）
