中国农学通报2026，Vol.42Issue(10)：1-5,5.
C离子束与组织培养相结合提高小麦诱变效率的研究
Research on Improving Wheat Mutagenesis Efficiency by Combining Carbon Ion Beam with Tissue Culture
摘要
Abstract
To investigate the effects of carbon(C)ion beam treatment on the tissue culture of wheat immature embryos and the somaclonal variation in their progeny,and to provide a reference for increasing the variation frequency of wheat somatic clones to address germplasm scarcity and accelerate new cultivar breeding,this study was conducted.Using the pure-line seed 15-676,which exhibits excellent agronomic traits but relatively tall plant height,immature embryos were treated with a carbon ion beam at a radiation dose of 80 Gy and then subjected to tissue culture.Untreated tissue-cultured embryos served as the control.The induction frequency,differentiation frequency,variations in major agronomic traits in the M2S1 and S1 generations,and the glutenin subunit composition were systematically examined.The results revealed that the differentiation frequency in the carbon ion beam-treated group was 11.6%,which was significantly lower than that in the control group(20.6%).In the M2S1 generation,the plant height in the carbon ion beam-treated group decreased by 11.74 cm compared to the control,a difference that was highly significant.Meanwhile,the treated group demonstrated increases in the number of grains per main spike,grain weight per main spike,and grain weight per plant.Additionally,both the M2S1 and S1 progeny grains retained the same high-quality glutenin subunits(5+10)as the original 15-676 variety.In conclusion,the combination of carbon ion beam treatment with tissue culture can effectively enhance the level of genetic variation in somatic clones,thereby expanding screening opportunities for beneficial mutants and providing abundant genetic materials for wheat germplasm innovation and new variety breeding.关键词
重离子/C离子束/小麦/辐射/诱变/组织培养/麦谷蛋白Key words
heavy ion/carbon ion beam/wheat/radiation/mutagenesis/tissue culture/glutenin分类
农业科技引用本文复制引用
孙岩,张宏纪,刘文林,唐婧泉,杨淑萍..C离子束与组织培养相结合提高小麦诱变效率的研究[J].中国农学通报,2026,42(10):1-5,5.基金项目
"十四五"国家重点研发计划项目"小麦定向诱变技术体系构建与新种质创制"(2022YFD1200701) （2022YFD1200701）
黑龙江省农业科学院创新工程资助项目"麦类作物突破性品种选育与产业化应用"(CX23GG03). （CX23GG03）
