中国农学通报2026，Vol.42Issue(10)：52-60,9.
'双丰1号'菜豆离体再生体系的建立
Establishment of in Vitro Regeneration System of'Shuang Feng No.1'Common Bean(Phaseolus vulgaris)
摘要
Abstract
Common bean(Phaseolus vulgaris)is an important legume vegetable,and its in vitro regeneration is difficult,which limits the efficiency of genetic transformation and molecular breeding.In order to establish an efficient and stable in vitro regeneration system of common bean,this study used'Shuangfeng No.1'common bean as material,cotyledonary nodes,hypocotyls,true leaves and cotyledons as explants,and used direct regeneration(callus pathway)and indirect regeneration(cotyledonary nodes directly bud)to optimize the ratio of plant growth regulators,low temperature and high osmotic stress conditions.The optimal parameters of callus induction,embryogenic callus differentiation,adventitious bud formation and rooting transplanting were systematically screened,and the in vitro regeneration system of common bean was established.The results showed that:(1)the best explants for loose callus induction were hypocotyls and cotyledonary nodes.The best medium was MS+1 mg/L 2,4-D,and the induction rates were 93.33%and 90.00%,respectively.(2)The optimal medium for inducing embryogenic callus in common bean(Phaseolus vulgaris)was MS+0.5 mg/L 2,4-D+0.25 mg/L 6-BA.Embryogenic callus was more easily induced when the explants were cultured at low temperature for 2 to 5 days.However,successful regeneration through callus was not achieved in this experiment,and further research is needed.(3)The indirect regeneration experiment indicated that the best explant was the cotyledon node,and the optimal induction medium was MS+2 mg/L 6-BA,with an adventitious bud induction rate of 100%.(4)The induced adventitious buds could be transferred to 1/2 MS+1 mg/L IBA rooting medium to regenerate complete plants.The rooting rate was 96.67%,the survival rate of transplanted seedlings was 84.16%,and the regenerated plants could flower and pod normally.This experiment established an efficient in vitro regeneration culture system for common bean(Phaseolus vulgaris),enabling rapid propagation of plants in vitro and laying the foundation for the establishment of a genetic transformation system for common bean(Phaseolus vulgaris).关键词
菜豆/松散型愈伤组织/胚性愈伤组织/子叶节再生/不定芽/植物生长调节剂Key words
common bean(Phaseolus vulgaris)/loose callus/embryogenic callus/cotyledons regeneration/adventitious buds/plant growth regulators分类
农业科技引用本文复制引用
许莹楠,刘艳军,黄俊轩,韩启厚,高逸卓,古瑜..'双丰1号'菜豆离体再生体系的建立[J].中国农学通报,2026,42(10):52-60,9.基金项目
天津市农业科学院财政种业创新研究项目 （）
天津市科技重大专项与工程计划项目"荚用菜豆新种质创制方法及种质资源创新研究"(17ZXZYNC00030). （17ZXZYNC00030）
