中国康复2026，Vol.41Issue(6)：323-329,7.DOI:10.3870/zgkf.2026.06.001
膝三针调节PPARγ/NF-κB通路对膝骨关节炎大鼠骨代谢及骨微结构的影响
The effects of knee three needles on bone metabolism and microstructure in knee osteoarthritis rats by regulating PPARγ/NF-κB pathway
摘要
Abstract
Objective:To explore the effects of knee three needles on bone metabolism and microstructure in knee osteoarthritis(KOA)rats by regulating the peroxisome proliferator-activated receptor gamma(PPARγ)/nuclear factor kappa B(NF-κB)pathway.Methods:Sodium iodoacetate solution was injected into the joint cavity to con-struct KOA rats.The successfully modeled rats were assigned into KOA group,knee three needles group(knee three needles treatment),and knee three needles+GW9662 group(knee three needles treatment+tail vein injection of 1 mg/kg GW9662),with 12 rats per group.There were 12 animals in the normal control(NC)group.The NC group and KOA group received intravenous in-jection of an equal amount of physiological saline once a day for 5 consecutive days per week,for a total of 3 weeks of treatment.The pain behavior of each group was tested[paw withdrawal threshold(PWT),paw withdrawal latency(PWL)].The enzyme-linked immunosorbent assay(ELISA)was used to detect the expression levels of tumor necrosis factor-α(TNF-α),interleukin(IL)-1β and IL-6,and bone metabolism indicators[type I collagen C-terminal peptide(CTX-Ⅰ),osteoprotegerin(OPG),tartrate-resistant acid phosphatase(TRACP),and receptor activator of nuclear factor κB ligand(RANKL)].Micro-CT was used to analyze the changes in the bone microstructure of the rat knee joint[including trabecular thickness(Tb.Th),trabecular number(Tb.N),structure model index(SMI),trabecular separation(Tb.Sp),bone mineral density(BMD),and bone volume/tissue volume ratio(BV/TV)].Hematoxylin and eosin(HE)staining and toluidine blue staining were implemented to measure pathological changes in knee cartilage tissue.In addition,Western blot was used to measure the expression of PPARγ/NF-κB pathway proteins in knee cartilage tissue.Results:The cells in each layer of cartilage tissue in the NC group were arranged neatly and distributed evenly.The surface of the cartilage tissue in the KOA group was un-even,with reduced cells,disordered arrangement,and a large number of vacuoles visible.The surface of the carti-lage tissue in the knee three needles group was relatively flat,with an increase in the number of cells and a decrease in vacuoles.The surface of the cartilage tissue in the knee three needles+GW9662 group was uneven,with a further decrease in cell number and an increase in vacuoles.Compared with the NC group,the KOA group showed decrea-ses in PWL,PWT,OPG,Tb.Th,Tb.N,BMD,BV/TV,and PPARγ,along with increases in Mankin score,CTX-Ⅰ,TRACP,RANKL,TNF-α,IL-1β,IL-6,SMI,Tb.Sp,and the ratio of p-NF-κB P65 to NF-κB P65(P<0.05).Compared with the KOA group,the knee three needles group exhibited increases in PWL,PWT,OPG,Tb.Th,Tb.N,BMD,BV/TV,and PPARγ,and decreases in Mankin score,CTX-Ⅰ,TRACP,RANKL,TNF-α,IL-1β,IL-6,SMI,Tb.Sp,and the p-NF-κB P65/NF-κB P65 ratio(P<0.05).The knee three needles+GW9662 group reversed the changes in the above factors induced by the knee three-needle acupuncture intervention(P<0.05).Conclusion:Knee acupuncture improves bone metabolism and microstructure in KOA rats by activating the PPARγ/NF-κB pathway.关键词
膝三针/过氧化物酶体增殖物激活受体γ/核因子κB/膝骨关节炎/骨代谢/骨微结构Key words
knee three needles/peroxisome proliferator-activated receptor gamma/nuclear factor kappa B/knee osteoarthritis/bone metabolism/bone microstructure分类
医药卫生引用本文复制引用
衣美霞,张蝶,王宁..膝三针调节PPARγ/NF-κB通路对膝骨关节炎大鼠骨代谢及骨微结构的影响[J].中国康复,2026,41(6):323-329,7.基金项目
2023年度军队中医药服务能力培育与提升专项计划(2023ZY025) （2023ZY025）
